Quantitative peptidomics: General considerations

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations

Abstract

Peptidomics is the detection and identification of the peptides present in a sample, while quantitative peptidomics provides additional information about the amounts of these peptides. Comparison of peptide levels among two or more samples is termed relative quantitation. It is also possible to perform absolute quantitation of peptide levels in which the biological sample is compared to synthetic standards, which requires a separate standard for each peptide. In contrast, relative quantitation can compare levels of all peptides that are detectable in a sample, which can exceed 1000 peptides in a complex sample. In this chapter, various techniques used for quantitative peptidomics are described along with discussion of the advantages and disadvantages of each approach. A guide to selecting the optimal quantitative approach is provided, based on the goals of the experiment and the resources that are available.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages121-140
Number of pages20
DOIs
StatePublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1719
ISSN (Print)1064-3745

Keywords

  • DiLeu
  • Formaldehyde
  • Isobaric tags
  • Isotopic label
  • iTRAQ
  • Label-free quantitation
  • TMAB

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Fricker, L. D. (2018). Quantitative peptidomics: General considerations. In Methods in Molecular Biology (pp. 121-140). (Methods in Molecular Biology; Vol. 1719). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7537-2_8