Quantitative nucleic acids footprinting

Thermodynamic and kinetic approaches

Victoria Petri, Michael D. Brenowitz

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Quantitative footprinting techniques allow a detailed analysis of the thermodynamic forces that characterize nucleic acid-ligand interactions and ligand-induced changes in nucleic acid structure by separately resolving the intrinsic and cooperative Gibbs free energy changes describing the reactions being investigated. A new implementation of the quantitative footprinting technique is the application of stopped-flow techniques to the study of kinetic reactions.

Original languageEnglish (US)
Pages (from-to)36-44
Number of pages9
JournalCurrent Opinion in Biotechnology
Volume8
Issue number1
DOIs
StatePublished - 1997

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Nucleic acids
Thermodynamics
Nucleic Acids
Ligands
Kinetics
Gibbs free energy
Reaction kinetics

ASJC Scopus subject areas

  • Biotechnology

Cite this

Quantitative nucleic acids footprinting : Thermodynamic and kinetic approaches. / Petri, Victoria; Brenowitz, Michael D.

In: Current Opinion in Biotechnology, Vol. 8, No. 1, 1997, p. 36-44.

Research output: Contribution to journalArticle

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