TY - JOUR
T1 - Quantitative analysis of the lipophlilic doxorubicin analogue annamycin in plasma and tissue samples by reversed‐phase chromatography
AU - Zou, Yiyu
AU - Hayman, Alan
AU - Priebe, Waldemar
AU - Perez‐Soler, Roman
N1 - Funding Information:
This work was supported in part by NIH CA-50270 and a grant from Argus Pharmaceuticals, Inc.
PY - 1993/11
Y1 - 1993/11
N2 - A rapid and sensitive HPLC method was developed to detect and quantitate the lipophilic doxorubicin analogue annamycin (Ann) and its metabolites in biological samples. Reversed‐phase chromatography coupled with fluorescence detection was used. The emission and excitation wavelengths of the fluorescent detector were set at 550 and 472 nm, respectively. The optimal mobile phase was acetonitrile:methanol:water at a 115:95:90 ratio (v/v/v). The lower limit of detection was 0.35 ng of Ann (7 ng/mL). The retention times of 4‐demethoxyadriamy‐cinone (Anone) and Ann were 2.6 and 4.3 min, respectively, and their resolution was 1.3. The precision of the determination of Ann and Anone were 0.5 ± 0.3 and 0.8 ± 0.7%, respectively. Ann dissolved in methanol was stable under the determination conditions. With chloroform, 60–90% of Ann was extracted from biological samples. Apart from Anone, two Ann metabolites (retention times 3.4 and 6.0 min) were detected in plasma and tissues from 05781/6 mice bearing subcutaneous B16 tumors 6 h after intravenous administration of a 5‐mg/kg suspension of Ann. No peaks were detected in blank tissues and plasma.
AB - A rapid and sensitive HPLC method was developed to detect and quantitate the lipophilic doxorubicin analogue annamycin (Ann) and its metabolites in biological samples. Reversed‐phase chromatography coupled with fluorescence detection was used. The emission and excitation wavelengths of the fluorescent detector were set at 550 and 472 nm, respectively. The optimal mobile phase was acetonitrile:methanol:water at a 115:95:90 ratio (v/v/v). The lower limit of detection was 0.35 ng of Ann (7 ng/mL). The retention times of 4‐demethoxyadriamy‐cinone (Anone) and Ann were 2.6 and 4.3 min, respectively, and their resolution was 1.3. The precision of the determination of Ann and Anone were 0.5 ± 0.3 and 0.8 ± 0.7%, respectively. Ann dissolved in methanol was stable under the determination conditions. With chloroform, 60–90% of Ann was extracted from biological samples. Apart from Anone, two Ann metabolites (retention times 3.4 and 6.0 min) were detected in plasma and tissues from 05781/6 mice bearing subcutaneous B16 tumors 6 h after intravenous administration of a 5‐mg/kg suspension of Ann. No peaks were detected in blank tissues and plasma.
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U2 - 10.1002/jps.2600821117
DO - 10.1002/jps.2600821117
M3 - Article
C2 - 8289131
AN - SCOPUS:0027132574
SN - 0022-3549
VL - 82
SP - 1151
EP - 1154
JO - Journal of Pharmaceutical Sciences
JF - Journal of Pharmaceutical Sciences
IS - 11
ER -