Quantitative analysis of the lipophlilic doxorubicin analogue annamycin in plasma and tissue samples by reversed‐phase chromatography

Yiyu Zou, Alan Hayman, Waldemar Priebe, Roman Perez‐Soler

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Abstract

A rapid and sensitive HPLC method was developed to detect and quantitate the lipophilic doxorubicin analogue annamycin (Ann) and its metabolites in biological samples. Reversed‐phase chromatography coupled with fluorescence detection was used. The emission and excitation wavelengths of the fluorescent detector were set at 550 and 472 nm, respectively. The optimal mobile phase was acetonitrile:methanol:water at a 115:95:90 ratio (v/v/v). The lower limit of detection was 0.35 ng of Ann (7 ng/mL). The retention times of 4‐demethoxyadriamy‐cinone (Anone) and Ann were 2.6 and 4.3 min, respectively, and their resolution was 1.3. The precision of the determination of Ann and Anone were 0.5 ± 0.3 and 0.8 ± 0.7%, respectively. Ann dissolved in methanol was stable under the determination conditions. With chloroform, 60–90% of Ann was extracted from biological samples. Apart from Anone, two Ann metabolites (retention times 3.4 and 6.0 min) were detected in plasma and tissues from 05781/6 mice bearing subcutaneous B16 tumors 6 h after intravenous administration of a 5‐mg/kg suspension of Ann. No peaks were detected in blank tissues and plasma.

Original languageEnglish (US)
Pages (from-to)1151-1154
Number of pages4
JournalJournal of Pharmaceutical Sciences
Volume82
Issue number11
DOIs
Publication statusPublished - Nov 1993
Externally publishedYes

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ASJC Scopus subject areas

  • Pharmaceutical Science

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