Quantitative analysis of the lipophilic doxorubicin analogue annamycin in plasma and tissue samples by reversed-phase chromatography

Yiyu Zou, A. Hayman, W. Priebe, Roman Perez-Soler

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

A rapid and sensitive HPLC method was developed to detect and quantitate the lipophilic doxorubicin analogue annamycin (Ann) and its metabolites in biological samples. Reversed-phase chromatography coupled with fluorescence detection was used. The emission and excitation wavelengths of the fluorescent detector were set at 550 and 472 nm, respectively. The optimal mobile phase was acetonitrile:methanol:water at a 115:95:90 ratio (v/v/v). The lower limit of detection was 0.35 ng of Ann (7 ng/mL). The retention times of 4-demethoxyadriamycinone (Anone) and Ann were 2.6 and 4.3 min, respectively, and their resolution was 1.3. The precision of the determination of Ann and Anone were 0.5 ± 0.3 and 0.8 ± 0.7%, respectively. Ann dissolved in methanol was stable under the determination conditions. With chloroform, 60-90% of Ann was extracted from biological samples. Apart from Anone, two Ann metabolites (retention times 3.4 and 6.0 min) were detected in plasma and tissues from C57BL/6 mice bearing subcutaneous B16 tumors 6 h after intravenous administration of a 5-mg/kg suspension of Ann. No peaks were detected in blank tissues and plasma.

Original languageEnglish (US)
Pages (from-to)1151-1154
Number of pages4
JournalJournal of Pharmaceutical Sciences
Volume82
Issue number11
DOIs
StatePublished - 1993
Externally publishedYes

Fingerprint

Reverse-Phase Chromatography
Chromatography
Doxorubicin
Tissue
Plasmas
Chemical analysis
Metabolites
Methanol
Bearings (structural)
annamycin
Chloroform
Inbred C57BL Mouse
Intravenous Administration
Limit of Detection
Tumors
Suspensions
Fluorescence
High Pressure Liquid Chromatography
Detectors
Wavelength

ASJC Scopus subject areas

  • Drug Discovery
  • Organic Chemistry
  • Chemistry(all)
  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science

Cite this

@article{e69cd958b47847e8b2b0b8857fe3e270,
title = "Quantitative analysis of the lipophilic doxorubicin analogue annamycin in plasma and tissue samples by reversed-phase chromatography",
abstract = "A rapid and sensitive HPLC method was developed to detect and quantitate the lipophilic doxorubicin analogue annamycin (Ann) and its metabolites in biological samples. Reversed-phase chromatography coupled with fluorescence detection was used. The emission and excitation wavelengths of the fluorescent detector were set at 550 and 472 nm, respectively. The optimal mobile phase was acetonitrile:methanol:water at a 115:95:90 ratio (v/v/v). The lower limit of detection was 0.35 ng of Ann (7 ng/mL). The retention times of 4-demethoxyadriamycinone (Anone) and Ann were 2.6 and 4.3 min, respectively, and their resolution was 1.3. The precision of the determination of Ann and Anone were 0.5 ± 0.3 and 0.8 ± 0.7{\%}, respectively. Ann dissolved in methanol was stable under the determination conditions. With chloroform, 60-90{\%} of Ann was extracted from biological samples. Apart from Anone, two Ann metabolites (retention times 3.4 and 6.0 min) were detected in plasma and tissues from C57BL/6 mice bearing subcutaneous B16 tumors 6 h after intravenous administration of a 5-mg/kg suspension of Ann. No peaks were detected in blank tissues and plasma.",
author = "Yiyu Zou and A. Hayman and W. Priebe and Roman Perez-Soler",
year = "1993",
doi = "10.1002/jps.2600821117",
language = "English (US)",
volume = "82",
pages = "1151--1154",
journal = "Journal of Pharmaceutical Sciences",
issn = "0022-3549",
publisher = "John Wiley and Sons Inc.",
number = "11",

}

TY - JOUR

T1 - Quantitative analysis of the lipophilic doxorubicin analogue annamycin in plasma and tissue samples by reversed-phase chromatography

AU - Zou, Yiyu

AU - Hayman, A.

AU - Priebe, W.

AU - Perez-Soler, Roman

PY - 1993

Y1 - 1993

N2 - A rapid and sensitive HPLC method was developed to detect and quantitate the lipophilic doxorubicin analogue annamycin (Ann) and its metabolites in biological samples. Reversed-phase chromatography coupled with fluorescence detection was used. The emission and excitation wavelengths of the fluorescent detector were set at 550 and 472 nm, respectively. The optimal mobile phase was acetonitrile:methanol:water at a 115:95:90 ratio (v/v/v). The lower limit of detection was 0.35 ng of Ann (7 ng/mL). The retention times of 4-demethoxyadriamycinone (Anone) and Ann were 2.6 and 4.3 min, respectively, and their resolution was 1.3. The precision of the determination of Ann and Anone were 0.5 ± 0.3 and 0.8 ± 0.7%, respectively. Ann dissolved in methanol was stable under the determination conditions. With chloroform, 60-90% of Ann was extracted from biological samples. Apart from Anone, two Ann metabolites (retention times 3.4 and 6.0 min) were detected in plasma and tissues from C57BL/6 mice bearing subcutaneous B16 tumors 6 h after intravenous administration of a 5-mg/kg suspension of Ann. No peaks were detected in blank tissues and plasma.

AB - A rapid and sensitive HPLC method was developed to detect and quantitate the lipophilic doxorubicin analogue annamycin (Ann) and its metabolites in biological samples. Reversed-phase chromatography coupled with fluorescence detection was used. The emission and excitation wavelengths of the fluorescent detector were set at 550 and 472 nm, respectively. The optimal mobile phase was acetonitrile:methanol:water at a 115:95:90 ratio (v/v/v). The lower limit of detection was 0.35 ng of Ann (7 ng/mL). The retention times of 4-demethoxyadriamycinone (Anone) and Ann were 2.6 and 4.3 min, respectively, and their resolution was 1.3. The precision of the determination of Ann and Anone were 0.5 ± 0.3 and 0.8 ± 0.7%, respectively. Ann dissolved in methanol was stable under the determination conditions. With chloroform, 60-90% of Ann was extracted from biological samples. Apart from Anone, two Ann metabolites (retention times 3.4 and 6.0 min) were detected in plasma and tissues from C57BL/6 mice bearing subcutaneous B16 tumors 6 h after intravenous administration of a 5-mg/kg suspension of Ann. No peaks were detected in blank tissues and plasma.

UR - http://www.scopus.com/inward/record.url?scp=0027132574&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027132574&partnerID=8YFLogxK

U2 - 10.1002/jps.2600821117

DO - 10.1002/jps.2600821117

M3 - Article

C2 - 8289131

AN - SCOPUS:0027132574

VL - 82

SP - 1151

EP - 1154

JO - Journal of Pharmaceutical Sciences

JF - Journal of Pharmaceutical Sciences

SN - 0022-3549

IS - 11

ER -