@inbook{d2c1826b746a41f59ed120452480427a,
title = "Quantification of HIV-1 gag localization within virus producer cells",
abstract = "Trafficking of newly synthesized Gag protein to the plasma membrane is one of the important steps during HIV-1 assembly. It requires participation of both viral and cellular determinants. Several techniques have been used to measure the amount of Gag that is associated with plasma membrane. Here we describe a microscopy-based method to estimate the distribution of Gag protein within the producer cell. This method can be used in conjunction with other biochemical techniques to quantify the distribution of Gag within a virus-producing cell and its accumulation at the plasma membrane. Since this method is microscopy based, it allows one to quantitate Gag across the cytoplasm, from the nuclear periphery to plasma membrane, at the single-cell level.",
keywords = "Gag trafficking, HIV-1 Gag, Quantitating Gag protein",
author = "Porte, {Annalena La} and Kalpana, {Ganjam V.}",
note = "Funding Information: This work was supported by NIH R01 GM112520 (to G.V.K.) and in part by Center for AIDS Research (CFAR) at the Albert Einstein College of Medicine funded by the NIH AI-051519. A.L. Was supported by the NIH funded Institutional AIDS training grant, T32-AI007501. We thank Dr. Jennifer Cano for initially optimizing the technique and Rosiris Leon for help with the experiment that generated the fi gure included in this chapter. Publisher Copyright: {\textcopyright} Springer Science+Business Media New York 2016.",
year = "2016",
doi = "10.1007/978-1-4939-3046-3_11",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "165--174",
booktitle = "Methods in Molecular Biology",
}
