Quantification of HIV-1 gag localization within virus producer cells

Annalena La Porte, Ganjam V. Kalpana

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations

Abstract

Trafficking of newly synthesized Gag protein to the plasma membrane is one of the important steps during HIV-1 assembly. It requires participation of both viral and cellular determinants. Several techniques have been used to measure the amount of Gag that is associated with plasma membrane. Here we describe a microscopy-based method to estimate the distribution of Gag protein within the producer cell. This method can be used in conjunction with other biochemical techniques to quantify the distribution of Gag within a virus-producing cell and its accumulation at the plasma membrane. Since this method is micros­copy based, it allows one to quantitate Gag across the cytoplasm, from the nuclear periphery to plasma membrane, at the single-cell level.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages165-174
Number of pages10
Volume1354
DOIs
StatePublished - 2016

Publication series

NameMethods in Molecular Biology
Volume1354
ISSN (Print)10643745

Keywords

  • Gag trafficking
  • HIV-1 Gag
  • Quantitating Gag protein

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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