Quantification of glutathione in Caenorhabditis elegans

Samuel W. Caito, Michael Aschner

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Glutathione (GSH) is the most abundant intracellular thiol with diverse functions from redox signaling, xenobiotic detoxification, and apoptosis. The quantification of GSH is an important measure for redox capacity and oxidative stress. This protocol quantifies total GSH from Caenorhabditis elegans, an emerging model organism for toxicology studies. GSH is measured using the 5,5'-dithiobis-(2-nitrobenzoic acid) (DTNB) cycling method originally created for cell and tissue samples but optimized for whole worm extracts. DTNB reacts with GSH to from a 5'-thio-2-nitrobenzoic acid (TNB) chromophore with maximum absorbance of 412 nm. This method is both rapid and sensitive, making it ideal for studies involving a large number of transgenic nematode strains.

Original languageEnglish (US)
Pages (from-to)6.18.1-6.18.6
JournalCurrent Protocols in Toxicology
Volume2015
DOIs
StatePublished - 2015

Fingerprint

Dithionitrobenzoic Acid
Caenorhabditis elegans
Glutathione
Oxidation-Reduction
Detoxification
Oxidative stress
Xenobiotics
Chromophores
Sulfhydryl Compounds
Toxicology
Oxidative Stress
Tissue
Apoptosis

Keywords

  • Caenorhabditis elegans
  • Glutathione

ASJC Scopus subject areas

  • Toxicology

Cite this

Quantification of glutathione in Caenorhabditis elegans. / Caito, Samuel W.; Aschner, Michael.

In: Current Protocols in Toxicology, Vol. 2015, 2015, p. 6.18.1-6.18.6.

Research output: Contribution to journalArticle

@article{04e031efd798410e9266fac7eb5e68da,
title = "Quantification of glutathione in Caenorhabditis elegans",
abstract = "Glutathione (GSH) is the most abundant intracellular thiol with diverse functions from redox signaling, xenobiotic detoxification, and apoptosis. The quantification of GSH is an important measure for redox capacity and oxidative stress. This protocol quantifies total GSH from Caenorhabditis elegans, an emerging model organism for toxicology studies. GSH is measured using the 5,5'-dithiobis-(2-nitrobenzoic acid) (DTNB) cycling method originally created for cell and tissue samples but optimized for whole worm extracts. DTNB reacts with GSH to from a 5'-thio-2-nitrobenzoic acid (TNB) chromophore with maximum absorbance of 412 nm. This method is both rapid and sensitive, making it ideal for studies involving a large number of transgenic nematode strains.",
keywords = "Caenorhabditis elegans, Glutathione",
author = "Caito, {Samuel W.} and Michael Aschner",
year = "2015",
doi = "10.1002/0471140856.tx0618s64",
language = "English (US)",
volume = "2015",
pages = "6.18.1--6.18.6",
journal = "Current protocols in toxicology / editorial board, Mahin D. Maines (editor-in-chief) ... [et al.]",
issn = "1934-9254",
publisher = "John Wiley and Sons Inc.",

}

TY - JOUR

T1 - Quantification of glutathione in Caenorhabditis elegans

AU - Caito, Samuel W.

AU - Aschner, Michael

PY - 2015

Y1 - 2015

N2 - Glutathione (GSH) is the most abundant intracellular thiol with diverse functions from redox signaling, xenobiotic detoxification, and apoptosis. The quantification of GSH is an important measure for redox capacity and oxidative stress. This protocol quantifies total GSH from Caenorhabditis elegans, an emerging model organism for toxicology studies. GSH is measured using the 5,5'-dithiobis-(2-nitrobenzoic acid) (DTNB) cycling method originally created for cell and tissue samples but optimized for whole worm extracts. DTNB reacts with GSH to from a 5'-thio-2-nitrobenzoic acid (TNB) chromophore with maximum absorbance of 412 nm. This method is both rapid and sensitive, making it ideal for studies involving a large number of transgenic nematode strains.

AB - Glutathione (GSH) is the most abundant intracellular thiol with diverse functions from redox signaling, xenobiotic detoxification, and apoptosis. The quantification of GSH is an important measure for redox capacity and oxidative stress. This protocol quantifies total GSH from Caenorhabditis elegans, an emerging model organism for toxicology studies. GSH is measured using the 5,5'-dithiobis-(2-nitrobenzoic acid) (DTNB) cycling method originally created for cell and tissue samples but optimized for whole worm extracts. DTNB reacts with GSH to from a 5'-thio-2-nitrobenzoic acid (TNB) chromophore with maximum absorbance of 412 nm. This method is both rapid and sensitive, making it ideal for studies involving a large number of transgenic nematode strains.

KW - Caenorhabditis elegans

KW - Glutathione

UR - http://www.scopus.com/inward/record.url?scp=84949232520&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84949232520&partnerID=8YFLogxK

U2 - 10.1002/0471140856.tx0618s64

DO - 10.1002/0471140856.tx0618s64

M3 - Article

VL - 2015

SP - 6.18.1-6.18.6

JO - Current protocols in toxicology / editorial board, Mahin D. Maines (editor-in-chief) ... [et al.]

JF - Current protocols in toxicology / editorial board, Mahin D. Maines (editor-in-chief) ... [et al.]

SN - 1934-9254

ER -