Quantification of genomic mutations in murine hematopoietic cells.

Hartmut Geiger, Schleimer David, Kalpana J. Nattamai, Jan Vijg

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Maintaining the stability of the genome is critical to cell survival and normal cell growth. Genetic modification of hematopoietic cells might bear an inherent increased risk for the accumulation of DNA mutations. It frequently requires cultivation of the cells under super-physiological oxygen levels, which can result in increased oxidative damage, as well as under super-physiological concentrations of cytokines, which might interfere with DNA-damage checkpoint activation and by this means might result in an increased mutational load. We describe here a protocol for monitoring the frequency of DNA mutations in bone marrow cells post transduction or upon selection either in vitro or in vivo based on the lacZ-plasmid (pUR288) transgenic mouse (small blue mouse) mutation indicator strain.

Original languageEnglish (US)
Pages (from-to)423-436
Number of pages14
JournalMethods in molecular biology (Clifton, N.J.)
Volume506
StatePublished - 2009
Externally publishedYes

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Mutation
Genomic Instability
DNA
Mutation Rate
Bone Marrow Cells
Transgenic Mice
DNA Damage
Cell Survival
Plasmids
Cytokines
Oxygen
Growth
Mutation Accumulation
In Vitro Techniques

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Quantification of genomic mutations in murine hematopoietic cells. / Geiger, Hartmut; David, Schleimer; Nattamai, Kalpana J.; Vijg, Jan.

In: Methods in molecular biology (Clifton, N.J.), Vol. 506, 2009, p. 423-436.

Research output: Contribution to journalArticle

Geiger, Hartmut ; David, Schleimer ; Nattamai, Kalpana J. ; Vijg, Jan. / Quantification of genomic mutations in murine hematopoietic cells. In: Methods in molecular biology (Clifton, N.J.). 2009 ; Vol. 506. pp. 423-436.
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