TY - JOUR
T1 - Purkinje Cell Collaterals Enable Output Signals from the Cerebellar Cortex to Feed Back to Purkinje Cells and Interneurons
AU - Witter, Laurens
AU - Rudolph, Stephanie
AU - Pressler, R. Todd
AU - Lahlaf, Safiya I.
AU - Regehr, Wade G.
N1 - Funding Information:
This work was supported by NIH R01NS032405 and R01NS092707; the Nancy Lurie Marks, Goldenson, Lefler, and Khodadad Foundations (W.G.R); a Goldenson and a Mahoney Fellowship to L.W.; a Brooks fellowship and an NIH F32NS087708 to S.R.; and NEI F32EY020718 to R.T.P. We thank the Image and Data Analysis Core (IDAC) and H.L. Elliott for help with image analysis, the Neurobiology Imaging Facility (NINDS P30 Core Center Grant NS072030), C. Hull and the W.G.R. lab for comments on the manuscript, and K. McDaniels for mouse colony maintenance.
Publisher Copyright:
© 2016 Elsevier Inc.
PY - 2016/7/20
Y1 - 2016/7/20
N2 - Purkinje cells (PCs) provide the sole output from the cerebellar cortex. Although PCs are well characterized on many levels, surprisingly little is known about their axon collaterals and their target neurons within the cerebellar cortex. It has been proposed that PC collaterals transiently control circuit assembly in early development, but it is thought that PC-to-PC connections are subsequently pruned. Here, we find that all PCs have collaterals in young, juvenile, and adult mice. Collaterals are restricted to the parasagittal plane, and most synapses are located in close proximity to PCs. Using optogenetics and electrophysiology, we find that in juveniles and adults, PCs make synapses onto other PCs, molecular layer interneurons, and Lugaro cells, but not onto Golgi cells. These findings establish that PC output can feed back and regulate numerous circuit elements within the cerebellar cortex and is well suited to contribute to processing in parasagittal zones.
AB - Purkinje cells (PCs) provide the sole output from the cerebellar cortex. Although PCs are well characterized on many levels, surprisingly little is known about their axon collaterals and their target neurons within the cerebellar cortex. It has been proposed that PC collaterals transiently control circuit assembly in early development, but it is thought that PC-to-PC connections are subsequently pruned. Here, we find that all PCs have collaterals in young, juvenile, and adult mice. Collaterals are restricted to the parasagittal plane, and most synapses are located in close proximity to PCs. Using optogenetics and electrophysiology, we find that in juveniles and adults, PCs make synapses onto other PCs, molecular layer interneurons, and Lugaro cells, but not onto Golgi cells. These findings establish that PC output can feed back and regulate numerous circuit elements within the cerebellar cortex and is well suited to contribute to processing in parasagittal zones.
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U2 - 10.1016/j.neuron.2016.05.037
DO - 10.1016/j.neuron.2016.05.037
M3 - Article
C2 - 27346533
AN - SCOPUS:84992059439
VL - 91
SP - 312
EP - 319
JO - Neuron
JF - Neuron
SN - 0896-6273
IS - 2
ER -