Purification of murine oncornaviral phosphoproteins using alkyl agarose derivatives..

S. L. Marcus, S. W. Smith, J. Racevskis, N. H. Sarkar

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Methods for the purification of both murine mammary tumor (type B) and murine leukemia (type C) oncornaviral phosphoproteins are described, in which chromatography on alkyl-agarose derivatives is used as the primary fractionation step. Gel filtration or ion exchange chromatography on phosphocellulose was the only subsequent step required for the purification of the type B and type C viral proteins, respectively. The two-step protocols also resulted in the co-purification of a low molecular weight core protein from each virus. Recoveries of the viral proteins purified by this method, based on per cent contribution of individual polypeptides to total virion proteins, were 70% or greater. Radioimmunocompetition analysis of the purified murine mammary tumor virus major core protein as well as analysis of the RNA binding properties of purified low molecular weight type C virus proteins suggests that neither antigenic reactivity nor specific RNA binding characteristics are altered by the purification protocols. The availability of these procedures should aid studies on the possible function and immunochemical properties of the native murine oncornaviral phosphoproteins and may also be extended to the purification of other oncornaviral proteins.

Original languageEnglish (US)
Pages (from-to)4809-4813
Number of pages5
JournalJournal of Biological Chemistry
Volume254
Issue number11
StatePublished - Jun 10 1979
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Purification of murine oncornaviral phosphoproteins using alkyl agarose derivatives..'. Together they form a unique fingerprint.

  • Cite this