Purification of Ligandin by Affinity Chromatography on Sulfobromophthalein-Agarose Gel

Allan W. Wolkoff, Madhu M. Bhargava, Cathie Chung, Zenaida Gatmaitan

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Abstract

Affinity chromatography of rat liver cytosol on sulfobromophthalein coupled to agarose via a glutathione bridge is described. Protein bound to the gel is eluted with 0.1 M Na2HPO4 at pH 9.2, resulting in a fourfold enrichment of ligandin as compared to cytosol. This technique should be useful in the study of organic anion binding to rat liver components other than cytosol, and may permit characterization of their potential role in hepatic uptake and excretory mechanisms.

Original languageEnglish (US)
Pages (from-to)150-153
Number of pages4
JournalProceedings of the Society for Experimental Biology and Medicine
Volume160
Issue number2
DOIs
Publication statusPublished - Feb 1979

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ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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