Purification of α-amylase from Bacillus licheniformis by chromatofocusing and gel filtration chromatography

M. Damodara Rao, A. Purnima, D. V. Ramesh, C. Ayyanna

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A combination of chromatofocusing and gel filtration chromatography resulted in a simple purification of α-amylase from Bacillus licheniformis. The purification was approximately 77-fold. Identification of the purity was established by SDS-PAGE. Molecular weight and isoelectric point of the purified enzyme were 58 kDa and 7.18 respectively. Western blot analysis confirms the specificity of antibody raised against purified α-amylase.

Original languageEnglish (US)
Pages (from-to)547-550
Number of pages4
JournalWorld Journal of Microbiology and Biotechnology
Issue number6
StatePublished - Aug 23 2002



  • Chromatofocusing
  • Gel filtration chromatography
  • α-Amylase

ASJC Scopus subject areas

  • Biotechnology
  • Physiology
  • Applied Microbiology and Biotechnology

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