Purification, characterization and crystallization of human platelet profilin expressed in Escherichia coli

A. A. Fedorov, T. D. Pollard, S. C. Almo

Research output: Contribution to journalArticlepeer-review

45 Scopus citations

Abstract

Human platelet profilin was expressed in Escherichia coli using a T7 based expression vector. The recombinant material is similar to authentic human platelet profilin based on the measured K(d) for rabbit skeletal muscle actin. Crystals of the recombinant material were obtained from both PEG 8000 and (NH4)2SO4. These crystals are isomorphous and belong to the monoclinic space group C2, a=75.0, b = 32.0, c = 62.5, β = 123°. These crystals contain one molecule in the asymmetric unit and diffract to at least 2.0 AÅ.

Original languageEnglish (US)
Pages (from-to)480-482
Number of pages3
JournalJournal of Molecular Biology
Volume241
Issue number3
DOIs
StatePublished - Jan 1 1994
Externally publishedYes

Keywords

  • Crystallization
  • Expression
  • Profilin, cytoskeleton
  • Protein structure

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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