Purification and Ex vivo expansion of fully functional salivary gland stem cells

Lalitha S.Y. Nanduri, Mirjam Baanstra, Hette Faber, Cecilia Rocchi, Erik Zwart, Gerald De Haan, Ronald Van Os, Robert P. Coppes

Research output: Contribution to journalArticlepeer-review

95 Scopus citations

Abstract

Hyposalivation often leads to irreversible and untreatable xerostomia. Salivary gland (SG) stem cell therapy is an attractive putative option to salvage these patients but is impeded by the limited availability of adult human tissue. Here, using murine SG cells, we demonstrate single-cell self-renewal, differentiation, enrichment of SG stem cells, and robust in vitro expansion. Dependent on stem cell marker expression, SG sphere-derived single cells could be differentiated in vitro into distinct lobular or ductal/lobular organoids, suggestive of progenitor or stem cell potency. Expanded cells were able to form miniglands/organoids containing multiple SG cell lineages. Expansion of these multipotent cells through serial passaging resulted in selection of a cell population, homogenous for stem cell marker expression (CD24hi/CD29hi). Cells highly expressing CD24 and CD29 could be prospectively isolated and were able to efficiently restore radiation-damaged SG function. Our approach will facilitate the use of adult SG stem cells for a variety of scientific and therapeutic purposes.

Original languageEnglish (US)
Pages (from-to)957-964
Number of pages8
JournalStem Cell Reports
Volume3
Issue number6
DOIs
StatePublished - Dec 9 2014
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Developmental Biology
  • Cell Biology

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