Purification and characterization of an extracellular endo-xylanase from an actinomycete Chainia sp.

Subray Hegde, A. R. Kumar, C. G. Suresh, S. M. Kotwal, K. N. Ganesh, M. I. Khan

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

An extracellular cellulase-free endo 1,4-β-D-xylanase from an actinomycete Chainia sp. has been purified to homogeneity by ammonium sulfate precipitation, successive chromatography on CM Sephadex C50 and Sephadex G50 followed by FPLC (M molecular mass of the enzyme indicates a single species with a mass of 21 000 and 21 485, as determined by SDS-PAGE and Laser Desorption Mass Spectroscopy, respectively. The purified xylanase is a non- glycosylated protein with pl of 9.4-9.5. The enzyme is low in basic, aromatic and sulfur containing amino acids and the partial N-terminal sequence exhibits remarkable homology with other actinomycete xylanases.

Original languageEnglish (US)
Pages (from-to)171-179
Number of pages9
JournalJournal of Biochemistry, Molecular Biology and Biophysics
Volume1
Issue number3
StatePublished - 1998
Externally publishedYes

Fingerprint

Actinobacteria
Purification
Endo-1,4-beta Xylanases
Sulfur Amino Acids
Cellulase
Ammonium Sulfate
Molecular mass
Enzymes
Chromatography
Sulfur
Polyacrylamide Gel Electrophoresis
Desorption
Mass Spectrometry
Lasers
Spectroscopy
Amino Acids
Proteins
sephadex

Keywords

  • Chainia sp.
  • Mode of action
  • Purification
  • Xylanase

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics
  • Biophysics

Cite this

Purification and characterization of an extracellular endo-xylanase from an actinomycete Chainia sp. / Hegde, Subray; Kumar, A. R.; Suresh, C. G.; Kotwal, S. M.; Ganesh, K. N.; Khan, M. I.

In: Journal of Biochemistry, Molecular Biology and Biophysics, Vol. 1, No. 3, 1998, p. 171-179.

Research output: Contribution to journalArticle

Hegde, Subray ; Kumar, A. R. ; Suresh, C. G. ; Kotwal, S. M. ; Ganesh, K. N. ; Khan, M. I. / Purification and characterization of an extracellular endo-xylanase from an actinomycete Chainia sp. In: Journal of Biochemistry, Molecular Biology and Biophysics. 1998 ; Vol. 1, No. 3. pp. 171-179.
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