TY - JOUR
T1 - PuraMatrix™ facilitates bone regeneration in bone defects of calvaria in mice
AU - Misawa, Haruo
AU - Kobayashi, Naoya
AU - Soto-Gutierrez, Alejandro
AU - Chen, Yong
AU - Yoshida, Aki
AU - Rivas-Carrillo, Jorge David
AU - Navarro-Alvarez, Nalu
AU - Tanaka, Kimiaki
AU - Miki, Atsushi
AU - Takei, Jiro
AU - Ueda, Tadayoshi
AU - Tanaka, Masato
AU - Endo, Hirosuke
AU - Tanaka, Noriaki
AU - Ozaki, Toshifumi
PY - 2006
Y1 - 2006
N2 - Artificial bones have often used for bone regeneration due to their strength, but they cannot provide an adequate environment for cell penetration and settlement. We therefore attempted to explore various materials that may allow the cells to penetrate and engraft in bone defects. PuraMatrix™ is a self-assembling peptide scaffold that produces a nanoscale environment allowing both cellular penetration and engraftment. The objective of this study was to investigate the effect of PuraMatrix™ on bone regeneration in a mouse bone defect model of the calvaria. Matrigel™ was used as a control. The expression of bone-related genes (alkaline phosphatase, Runx2, and Osterix) in the PuraMatrix™-injected bone defects was stronger than that in the Matrigel™-injected defects. Soft X-ray radiographs revealed that bony bridges were clearly observed in the defects treated with PuraMatrix™, but not in the Matrigel™-treated defects. Notably, PuraMatrix™ treatment induced mature bone tissue while showing cortical bone medullary cavities. The area of newly formed bones at the site of the bone defects was 1.38-fold larger for PuraMatrix™ than Matrigel™. The strength of the regenerated bone was 1.72-fold higher for PuraMatrix™ (146.0 g) than for Matrigel™ (84.7 g). The present study demonstrated that PuraMatrix™ injection favorably induced functional bone regeneration.
AB - Artificial bones have often used for bone regeneration due to their strength, but they cannot provide an adequate environment for cell penetration and settlement. We therefore attempted to explore various materials that may allow the cells to penetrate and engraft in bone defects. PuraMatrix™ is a self-assembling peptide scaffold that produces a nanoscale environment allowing both cellular penetration and engraftment. The objective of this study was to investigate the effect of PuraMatrix™ on bone regeneration in a mouse bone defect model of the calvaria. Matrigel™ was used as a control. The expression of bone-related genes (alkaline phosphatase, Runx2, and Osterix) in the PuraMatrix™-injected bone defects was stronger than that in the Matrigel™-injected defects. Soft X-ray radiographs revealed that bony bridges were clearly observed in the defects treated with PuraMatrix™, but not in the Matrigel™-treated defects. Notably, PuraMatrix™ treatment induced mature bone tissue while showing cortical bone medullary cavities. The area of newly formed bones at the site of the bone defects was 1.38-fold larger for PuraMatrix™ than Matrigel™. The strength of the regenerated bone was 1.72-fold higher for PuraMatrix™ (146.0 g) than for Matrigel™ (84.7 g). The present study demonstrated that PuraMatrix™ injection favorably induced functional bone regeneration.
KW - Bone regeneration
KW - Calvaria
KW - Matrigel™
KW - PuraMatrix™
UR - http://www.scopus.com/inward/record.url?scp=33846597141&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33846597141&partnerID=8YFLogxK
U2 - 10.3727/000000006783981369
DO - 10.3727/000000006783981369
M3 - Article
C2 - 17299995
AN - SCOPUS:33846597141
SN - 0963-6897
VL - 15
SP - 903
EP - 910
JO - Cell Transplantation
JF - Cell Transplantation
IS - 10
ER -