Pulsed Electron-Nuclear Double Resonance (ENDOR) at 140 GHz

M. Bennati, C. T. Farrar, J. A. Bryant, S. J. Inati, V. Weis, Gary J. Gerfen, P. Riggs-Gelasco, J. Stubbe, R. G. Griffin

Research output: Contribution to journalArticle

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Abstract

We describe a spectrometer for pulsed ENDOR at 140 GHz, which is based on microwave IMPATT diode amplifiers and a probe consisting of a TE011 cavity with a high-quality resonance circuit for variable radiofrequency irradiation. For pulsed EPR we obtain an absolute sensitivity of 3 × 109 spins/Gauss at 20 K. The performance of the spectrometer is demonstrated with pulsed ENDOR spectra of a standard bis-diphenylene-phenyl-allyl (BDPA) doped into polystyrene and of the tyrosyl radical from E. coli ribonucleotide reductase (RNR). The EPR spectrum of the RNR tyrosyl radical displays substantial g-anisotropy at 5 T and is used to demonstrate orientation-selective Davies-ENDOR.

Original languageEnglish (US)
Pages (from-to)232-243
Number of pages12
JournalJournal of Magnetic Resonance
Volume138
Issue number2
StatePublished - Jun 1999

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Electron Spin Resonance Spectroscopy
Ribonucleotide Reductases
Paramagnetic resonance
Spectrometers
Electrons
Diode amplifiers
Circuit resonance
IMPATT diodes
electrons
Polystyrenes
Anisotropy
spectrometers
Microwaves
avalanche diodes
Escherichia coli
Irradiation
polystyrene
amplifiers
microwaves
anisotropy

ASJC Scopus subject areas

  • Molecular Biology
  • Physical and Theoretical Chemistry
  • Spectroscopy
  • Radiology Nuclear Medicine and imaging
  • Condensed Matter Physics

Cite this

Bennati, M., Farrar, C. T., Bryant, J. A., Inati, S. J., Weis, V., Gerfen, G. J., ... Griffin, R. G. (1999). Pulsed Electron-Nuclear Double Resonance (ENDOR) at 140 GHz. Journal of Magnetic Resonance, 138(2), 232-243.

Pulsed Electron-Nuclear Double Resonance (ENDOR) at 140 GHz. / Bennati, M.; Farrar, C. T.; Bryant, J. A.; Inati, S. J.; Weis, V.; Gerfen, Gary J.; Riggs-Gelasco, P.; Stubbe, J.; Griffin, R. G.

In: Journal of Magnetic Resonance, Vol. 138, No. 2, 06.1999, p. 232-243.

Research output: Contribution to journalArticle

Bennati, M, Farrar, CT, Bryant, JA, Inati, SJ, Weis, V, Gerfen, GJ, Riggs-Gelasco, P, Stubbe, J & Griffin, RG 1999, 'Pulsed Electron-Nuclear Double Resonance (ENDOR) at 140 GHz', Journal of Magnetic Resonance, vol. 138, no. 2, pp. 232-243.
Bennati M, Farrar CT, Bryant JA, Inati SJ, Weis V, Gerfen GJ et al. Pulsed Electron-Nuclear Double Resonance (ENDOR) at 140 GHz. Journal of Magnetic Resonance. 1999 Jun;138(2):232-243.
Bennati, M. ; Farrar, C. T. ; Bryant, J. A. ; Inati, S. J. ; Weis, V. ; Gerfen, Gary J. ; Riggs-Gelasco, P. ; Stubbe, J. ; Griffin, R. G. / Pulsed Electron-Nuclear Double Resonance (ENDOR) at 140 GHz. In: Journal of Magnetic Resonance. 1999 ; Vol. 138, No. 2. pp. 232-243.
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