Pulsed Electron-Nuclear Double Resonance (ENDOR) at 140 GHz

M. Bennati, C. T. Farrar, J. A. Bryant, S. J. Inati, V. Weis, G. J. Gerfen, P. Riggs-Gelasco, J. Stubbe, R. G. Griffin

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83 Scopus citations

Abstract

We describe a spectrometer for pulsed ENDOR at 140 GHz, which is based on microwave IMPATT diode amplifiers and a probe consisting of a TE011 cavity with a high-quality resonance circuit for variable radiofrequency irradiation. For pulsed EPR we obtain an absolute sensitivity of 3 × 109 spins/Gauss at 20 K. The performance of the spectrometer is demonstrated with pulsed ENDOR spectra of a standard bis-diphenylene-phenyl-allyl (BDPA) doped into polystyrene and of the tyrosyl radical from E. coli ribonucleotide reductase (RNR). The EPR spectrum of the RNR tyrosyl radical displays substantial g-anisotropy at 5 T and is used to demonstrate orientation-selective Davies-ENDOR.

Original languageEnglish (US)
Pages (from-to)232-243
Number of pages12
JournalJournal of Magnetic Resonance
Volume138
Issue number2
DOIs
StatePublished - Jun 1999

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Nuclear and High Energy Physics
  • Condensed Matter Physics

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