PU.1 expression is modulated by the balance of functional sense and antisense RNAs regulated by a shared cis-regulatory element

Alexander K. Ebralidze; Florence C. Guibal; Ulrich Steidl; Pu Zhang; Sanghoon Lee; Boris Bartholdy; Meritxell Alberich Jorda; Victoria Petkova; Frank Rosenbauer; Gang Huang; Tajhal Dayaram; Johanna Klupp; Karen B. O'Brien; Britta Will; Maarten Hoogenkamp; Katherine L.B. Borden; Constanze Bonifer; Daniel G. Tenen

doi:10.1101/gad.1654808

PU.1 expression is modulated by the balance of functional sense and antisense RNAs regulated by a shared cis-regulatory element

Alexander K. Ebralidze, Florence C. Guibal, Ulrich Steidl, Pu Zhang, Sanghoon Lee, Boris Bartholdy, Meritxell Alberich Jorda, Victoria Petkova, Frank Rosenbauer, Gang Huang, Tajhal Dayaram, Johanna Klupp, Karen B. O'Brien, Britta Will, Maarten Hoogenkamp, Katherine L.B. Borden, Constanze Bonifer, Daniel G. Tenen

Cell Biology

Research output: Contribution to journal › Article › peer-review

149 Scopus citations

Abstract

The transcription factor PU.1 is an important regulator of hematopoiesis; precise expression levels are critical for normal hematopoietic development and suppression of leukemia. We show here that noncoding antisense RNAs are important modulators of proper dosages of PU.1. Antisense and sense RNAs are regulated by shared evolutionarily conserved cis-regulatory elements, and we can show that antisense RNAs inhibit PU.1 expression by modulating mRNA translation. We propose that such antisense RNAs will likely be important in the regulation of many genes and may be the reason for the large number of overlapping complementary transcripts with so far unknown function.

Original language	English (US)
Pages (from-to)	2085-2092
Number of pages	8
Journal	Genes and Development
Volume	22
Issue number	15
DOIs	https://doi.org/10.1101/gad.1654808
State	Published - Aug 1 2008

Keywords

Coordinated expression of the target and regulator
Noncoding antisense RNA
Translation stalling
Upstream and intronic regulatory elements

ASJC Scopus subject areas

Genetics
Developmental Biology

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10.1101/gad.1654808

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Ebralidze, A. K., Guibal, F. C., Steidl, U., Zhang, P., Lee, S., Bartholdy, B., Jorda, M. A., Petkova, V., Rosenbauer, F., Huang, G., Dayaram, T., Klupp, J., O'Brien, K. B., Will, B., Hoogenkamp, M., Borden, K. L. B., Bonifer, C., & Tenen, D. G. (2008). PU.1 expression is modulated by the balance of functional sense and antisense RNAs regulated by a shared cis-regulatory element. Genes and Development, 22(15), 2085-2092. https://doi.org/10.1101/gad.1654808

Ebralidze, AK, Guibal, FC, Steidl, U, Zhang, P, Lee, S, Bartholdy, B, Jorda, MA, Petkova, V, Rosenbauer, F, Huang, G, Dayaram, T, Klupp, J, O'Brien, KB, Will, B, Hoogenkamp, M, Borden, KLB, Bonifer, C & Tenen, DG 2008, 'PU.1 expression is modulated by the balance of functional sense and antisense RNAs regulated by a shared cis-regulatory element', Genes and Development, vol. 22, no. 15, pp. 2085-2092. https://doi.org/10.1101/gad.1654808

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abstract = "The transcription factor PU.1 is an important regulator of hematopoiesis; precise expression levels are critical for normal hematopoietic development and suppression of leukemia. We show here that noncoding antisense RNAs are important modulators of proper dosages of PU.1. Antisense and sense RNAs are regulated by shared evolutionarily conserved cis-regulatory elements, and we can show that antisense RNAs inhibit PU.1 expression by modulating mRNA translation. We propose that such antisense RNAs will likely be important in the regulation of many genes and may be the reason for the large number of overlapping complementary transcripts with so far unknown function.",

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AU - Zhang, Pu

AU - Lee, Sanghoon

AU - Bartholdy, Boris

AU - Jorda, Meritxell Alberich

AU - Petkova, Victoria

AU - Rosenbauer, Frank

AU - Huang, Gang

AU - Dayaram, Tajhal

AU - Klupp, Johanna

AU - O'Brien, Karen B.

AU - Will, Britta

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KW - Translation stalling

KW - Upstream and intronic regulatory elements

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PU.1 expression is modulated by the balance of functional sense and antisense RNAs regulated by a shared cis-regulatory element

Abstract

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