TY - JOUR
T1 - PTP1B-dependent regulation of receptor tyrosine kinase signaling by the actin-binding protein Mena
AU - Hughes, Shannon K.
AU - Oudin, Madeleine J.
AU - Tadros, Jenny
AU - Neil, Jason
AU - Del Rosario, Amanda
AU - Joughin, Brian A.
AU - Ritsma, Laila
AU - Wyckoff, Jeff
AU - Vasile, Eliza
AU - Eddy, Robert
AU - Philippar, Ulrike
AU - Lussiez, Alisha
AU - Condeelis, John S.
AU - Van Rheenen, Jacco
AU - White, Forest
AU - Lauffenburger, Douglas A.
AU - Gertler, Frank B.
N1 - Publisher Copyright:
© 2015 Hughes, Oudin, et al.
PY - 2015/11/1
Y1 - 2015/11/1
N2 - During breast cancer progression, alternative mRNA splicing produces functionally distinct isoforms of Mena, an actin regulator with roles in cell migration and metastasis. Aggressive tumor cell subpopulations express MenaINV, which promotes tumor cell invasion by potentiating EGF responses. However, the mechanism by which this occurs is unknown. Here we report that Mena associates constitutively with the tyrosine phosphatase PTP1B and mediates a novel negative feedback mechanism that attenuates receptor tyrosine kinase signaling. On EGF stimulation, complexes containing Mena and PTP1B are recruited to the EGFR, causing receptor dephosphorylation and leading to decreased motility responses. Mena also interacts with the 5′ inositol phosphatase SHIP2, which is important for the recruitment of the Mena-PTP1B complex to the EGFR. When MenaINV is expressed, PTP1B recruitment to the EGFR is impaired, providing a mechanism for growth factor sensitization to EGF, as well as HGF and IGF, and increased resistance to EGFR and Met inhibitors in signaling and motility assays. In sum, we demonstrate that Mena plays an important role in regulating growth factor-induced signaling. Disruption of this attenuation by MenaINV sensitizes tumor cells to low-growth factor concentrations, thereby increasing the migration and invasion responses that contribute to aggressive, malignant cell phenotypes.
AB - During breast cancer progression, alternative mRNA splicing produces functionally distinct isoforms of Mena, an actin regulator with roles in cell migration and metastasis. Aggressive tumor cell subpopulations express MenaINV, which promotes tumor cell invasion by potentiating EGF responses. However, the mechanism by which this occurs is unknown. Here we report that Mena associates constitutively with the tyrosine phosphatase PTP1B and mediates a novel negative feedback mechanism that attenuates receptor tyrosine kinase signaling. On EGF stimulation, complexes containing Mena and PTP1B are recruited to the EGFR, causing receptor dephosphorylation and leading to decreased motility responses. Mena also interacts with the 5′ inositol phosphatase SHIP2, which is important for the recruitment of the Mena-PTP1B complex to the EGFR. When MenaINV is expressed, PTP1B recruitment to the EGFR is impaired, providing a mechanism for growth factor sensitization to EGF, as well as HGF and IGF, and increased resistance to EGFR and Met inhibitors in signaling and motility assays. In sum, we demonstrate that Mena plays an important role in regulating growth factor-induced signaling. Disruption of this attenuation by MenaINV sensitizes tumor cells to low-growth factor concentrations, thereby increasing the migration and invasion responses that contribute to aggressive, malignant cell phenotypes.
UR - http://www.scopus.com/inward/record.url?scp=84945950866&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84945950866&partnerID=8YFLogxK
U2 - 10.1091/mbc.E15-06-0442
DO - 10.1091/mbc.E15-06-0442
M3 - Article
C2 - 26337385
AN - SCOPUS:84945950866
SN - 1059-1524
VL - 26
SP - 3867
EP - 3878
JO - Molecular biology of the cell
JF - Molecular biology of the cell
IS - 21
ER -