PTEN in neural precursor cells

Regulation of migration, apoptosis, and proliferation

Li Li, Fenghua Liu, Rebecca A. Salmonsen, Tod K. Turner, N. Scott Litofsky, Antonio Di Cristofano, Pier Paolo Pandolfi, Stephen N. Jones, Larry D. Recht, Alonzo H. Ross

Research output: Contribution to journalArticle

104 Citations (Scopus)

Abstract

PTEN is a lipid phosphatase, and PTEN mutations are associated with gliomas, macrocephaly, and mental deficiencies. We have used PTEN +/- mice to assess PTEN's role in subventricular zone (SVZ) precursor cells. For cultured SVZ neurosphere cells, haploinsufficiency for PTEN increases phosphorylation of Akt and forkhead transcription factor and slightly enhances proliferation. Based on a filter penetration assay, PTEN +/- cells are substantially more migratory and invasive than +/+ cells. The +/- cells also are more resistant to H2O2-induced apoptosis. Analysis of PTEN +/- and +/+ mice by BrdU labeling reveals no difference in the rate of cell proliferation in the SVZ. Exit of BrdU-labeled cells from the SVZ and radial migration to the outer layers of the olfactory bulb are more rapid for +/- cells. These observations indicate that PTEN regulates SVZ precursor cell function and is particularly important for migration and apoptosis in response to oxidative stress.

Original languageEnglish (US)
Pages (from-to)21-29
Number of pages9
JournalMolecular and Cellular Neuroscience
Volume20
Issue number1
DOIs
StatePublished - 2002
Externally publishedYes

Fingerprint

Cell Movement
Lateral Ventricles
Apoptosis
Bromodeoxyuridine
PTEN Phosphohydrolase
Megalencephaly
Forkhead Transcription Factors
Haploinsufficiency
Olfactory Bulb
Glioma
Intellectual Disability
Oxidative Stress
Phosphorylation
Cell Proliferation
Lipids
Mutation

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Developmental Neuroscience

Cite this

PTEN in neural precursor cells : Regulation of migration, apoptosis, and proliferation. / Li, Li; Liu, Fenghua; Salmonsen, Rebecca A.; Turner, Tod K.; Litofsky, N. Scott; Di Cristofano, Antonio; Pandolfi, Pier Paolo; Jones, Stephen N.; Recht, Larry D.; Ross, Alonzo H.

In: Molecular and Cellular Neuroscience, Vol. 20, No. 1, 2002, p. 21-29.

Research output: Contribution to journalArticle

Li, L, Liu, F, Salmonsen, RA, Turner, TK, Litofsky, NS, Di Cristofano, A, Pandolfi, PP, Jones, SN, Recht, LD & Ross, AH 2002, 'PTEN in neural precursor cells: Regulation of migration, apoptosis, and proliferation', Molecular and Cellular Neuroscience, vol. 20, no. 1, pp. 21-29. https://doi.org/10.1006/mcne.2002.1115
Li, Li ; Liu, Fenghua ; Salmonsen, Rebecca A. ; Turner, Tod K. ; Litofsky, N. Scott ; Di Cristofano, Antonio ; Pandolfi, Pier Paolo ; Jones, Stephen N. ; Recht, Larry D. ; Ross, Alonzo H. / PTEN in neural precursor cells : Regulation of migration, apoptosis, and proliferation. In: Molecular and Cellular Neuroscience. 2002 ; Vol. 20, No. 1. pp. 21-29.
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