TY - JOUR
T1 - PTEN in neural precursor cells
T2 - Regulation of migration, apoptosis, and proliferation
AU - Li, Li
AU - Liu, Fenghua
AU - Salmonsen, Rebecca A.
AU - Turner, Tod K.
AU - Litofsky, N. Scott
AU - Di Cristofano, Antonio
AU - Pandolfi, Pier Paolo
AU - Jones, Stephen N.
AU - Recht, Larry D.
AU - Ross, Alonzo H.
N1 - Funding Information:
We thank T. Jang, J. Labrie, R. Gerstein, M. Mitome, Y. Xue, B. Greco, J. Lian, the UMASS Flow Cytometry Center, and P. Quesen-berry for their help in these studies and R. Ren for the retroviral vector. This work was supported by NIH Grants NS21716 and CA68426.
PY - 2002
Y1 - 2002
N2 - PTEN is a lipid phosphatase, and PTEN mutations are associated with gliomas, macrocephaly, and mental deficiencies. We have used PTEN +/- mice to assess PTEN's role in subventricular zone (SVZ) precursor cells. For cultured SVZ neurosphere cells, haploinsufficiency for PTEN increases phosphorylation of Akt and forkhead transcription factor and slightly enhances proliferation. Based on a filter penetration assay, PTEN +/- cells are substantially more migratory and invasive than +/+ cells. The +/- cells also are more resistant to H2O2-induced apoptosis. Analysis of PTEN +/- and +/+ mice by BrdU labeling reveals no difference in the rate of cell proliferation in the SVZ. Exit of BrdU-labeled cells from the SVZ and radial migration to the outer layers of the olfactory bulb are more rapid for +/- cells. These observations indicate that PTEN regulates SVZ precursor cell function and is particularly important for migration and apoptosis in response to oxidative stress.
AB - PTEN is a lipid phosphatase, and PTEN mutations are associated with gliomas, macrocephaly, and mental deficiencies. We have used PTEN +/- mice to assess PTEN's role in subventricular zone (SVZ) precursor cells. For cultured SVZ neurosphere cells, haploinsufficiency for PTEN increases phosphorylation of Akt and forkhead transcription factor and slightly enhances proliferation. Based on a filter penetration assay, PTEN +/- cells are substantially more migratory and invasive than +/+ cells. The +/- cells also are more resistant to H2O2-induced apoptosis. Analysis of PTEN +/- and +/+ mice by BrdU labeling reveals no difference in the rate of cell proliferation in the SVZ. Exit of BrdU-labeled cells from the SVZ and radial migration to the outer layers of the olfactory bulb are more rapid for +/- cells. These observations indicate that PTEN regulates SVZ precursor cell function and is particularly important for migration and apoptosis in response to oxidative stress.
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U2 - 10.1006/mcne.2002.1115
DO - 10.1006/mcne.2002.1115
M3 - Article
C2 - 12056837
AN - SCOPUS:0036279763
SN - 1044-7431
VL - 20
SP - 21
EP - 29
JO - Molecular and Cellular Neuroscience
JF - Molecular and Cellular Neuroscience
IS - 1
ER -