Prrx1 Fibroblasts Represent a Pro-fibrotic Lineage in the Mouse Ventral Dermis

Tripp Leavitt, Michael S. Hu, Mimi R. Borrelli, Michael Januszyk, Julia T. Garcia, Ryan C. Ransom, Shamik Mascharak, Heather E. desJardins-Park, Ulrike M. Litzenburger, Graham G. Walmsley, Clement D. Marshall, Alessandra L. Moore, Bryan Duoto, Sandeep Adem, Deshka S. Foster, Ankit Salhotra, Abra H. Shen, Michelle Griffin, Ethan Z. Shen, Leandra A. BarnesElizabeth R. Zielins, Zeshaan N. Maan, Yuning Wei, Charles K.F. Chan, Derrick C. Wan, Hermann P. Lorenz, Howard Y. Chang, Geoffrey C. Gurtner, Michael T. Longaker

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Fibroblast heterogeneity has been shown within the unwounded mouse dorsal dermis, with fibroblast subpopulations being identified according to anatomical location and embryonic lineage. Using lineage tracing, we demonstrate that paired related homeobox 1 (Prrx1)-expressing fibroblasts are responsible for acute and chronic fibroses in the ventral dermis. Single-cell transcriptomics further corroborated the inherent fibrotic characteristics of Prrx1 fibroblasts during wound repair. In summary, we identify and characterize a fibroblast subpopulation in the mouse ventral dermis with intrinsic scar-forming potential.

Original languageEnglish (US)
Article number108356
JournalCell Reports
Volume33
Issue number6
DOIs
StatePublished - Nov 10 2020
Externally publishedYes

Keywords

  • cell barcoding
  • dermis
  • fibroblast heterogeneity
  • fibroblasts
  • fibrosis
  • paired related homeobox 1
  • Prrx-1
  • radiation-induced fibrosis
  • RNA sequencing
  • RNA-seq
  • scarring
  • single-cell RNA sequencing
  • transcriptomics
  • tumor stroma formation
  • wound healing

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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