@article{76c1d8c8edb74f3f929273c95c4caa47,
title = "Prrx1 Fibroblasts Represent a Pro-fibrotic Lineage in the Mouse Ventral Dermis",
abstract = "Fibroblast heterogeneity has been shown within the unwounded mouse dorsal dermis, with fibroblast subpopulations being identified according to anatomical location and embryonic lineage. Using lineage tracing, we demonstrate that paired related homeobox 1 (Prrx1)-expressing fibroblasts are responsible for acute and chronic fibroses in the ventral dermis. Single-cell transcriptomics further corroborated the inherent fibrotic characteristics of Prrx1 fibroblasts during wound repair. In summary, we identify and characterize a fibroblast subpopulation in the mouse ventral dermis with intrinsic scar-forming potential.",
keywords = "Prrx-1, RNA sequencing, RNA-seq, cell barcoding, dermis, fibroblast heterogeneity, fibroblasts, fibrosis, paired related homeobox 1, radiation-induced fibrosis, scarring, single-cell RNA sequencing, transcriptomics, tumor stroma formation, wound healing",
author = "Tripp Leavitt and Hu, {Michael S.} and Borrelli, {Mimi R.} and Michael Januszyk and Garcia, {Julia T.} and Ransom, {Ryan C.} and Shamik Mascharak and desJardins-Park, {Heather E.} and Litzenburger, {Ulrike M.} and Walmsley, {Graham G.} and Marshall, {Clement D.} and Moore, {Alessandra L.} and Bryan Duoto and Sandeep Adem and Foster, {Deshka S.} and Ankit Salhotra and Shen, {Abra H.} and Michelle Griffin and Shen, {Ethan Z.} and Barnes, {Leandra A.} and Zielins, {Elizabeth R.} and Maan, {Zeshaan N.} and Yuning Wei and Chan, {Charles K.F.} and Wan, {Derrick C.} and Lorenz, {Hermann P.} and Chang, {Howard Y.} and Gurtner, {Geoffrey C.} and Longaker, {Michael T.}",
note = "Funding Information: We would like to acknowledge Patty Lovelace and Catherine Crumpton in the Shared FACS Facility in the Lokey Stem Cell Institute, the Dauskardt lab at Stanford University for use of microtester, and Vida Shokoohi at the Stanford Functional Genomics Facility for technical help with sequencing library preparation. This work was supported by the Hagey Laboratory for Pediatric Regenerative Medicine; the Gunn/Olivier Research Fund; the Stinehart-Reed Fund (to M.T.L.); NIH grants R01 GM116892 (to H.P.L. and M.T.L.), P50-HG007735 (to H.Y.C.), R0I DE 027346 (to D.C.W.), and R01 GM136659 (to M.T.L.); the Scleroderma Research Foundation (to H.Y.C.); the Plastic Surgery Research Foundation (to M.R.B.); the U.K. Fullbright Commission (to M.G.); the Sarnoff Cardiovascular Research Foundation (to A.H.S.); and F.P. Johnson, Jr. (to H.Y.C. and M.T.L.). H.Y.C. is an investigator of the Howard Hughes Medical Institute. T.L. M.R.B. M.G. and M.J. conceived, designed, and oversaw experiments, with suggestions from M.S.H. R.C.R. and M.T.L. T.L. M.S.H. M.R.B. M.J. J.T.G. M.G. R.C.R. S.M. and L.A.B. performed experiments and analyzed data. M.J. performed 10x genomic analysis. G.G.W. assisted with experimental design. C.D.M. A.L.M. A.H.S. H.E.d.-P. U.M.L. B.D. S.A. E.Z.S. L.A.B. Z.N.M. Y.W. and E.R.Z. assisted with data collection. M.R.B. M.G. R.C.R. T.L. M.S.H. and M.J. wrote the manuscript. C.K.F.C. D.C.W. P.H.L. H.Y.C. G.C.G. and M.T.L. edited the manuscript. H.Y.C. is a co-founder of Accent Therapeutics and Boundless Bio and an advisor to 10x Genomics, Arsenal Bioscience, and Spring Discovery. Funding Information: We would like to acknowledge Patty Lovelace and Catherine Crumpton in the Shared FACS Facility in the Lokey Stem Cell Institute, the Dauskardt lab at Stanford University for use of microtester, and Vida Shokoohi at the Stanford Functional Genomics Facility for technical help with sequencing library preparation. This work was supported by the Hagey Laboratory for Pediatric Regenerative Medicine ; the Gunn/Olivier Research Fund ; the Stinehart-Reed Fund (to M.T.L.); NIH grants R01 GM116892 (to H.P.L. and M.T.L.), P50-HG007735 (to H.Y.C.), R0I DE 027346 (to D.C.W.), and R01 GM136659 (to M.T.L.); the Scleroderma Research Foundation (to H.Y.C.); the Plastic Surgery Research Foundation (to M.R.B.); the U.K. Fullbright Commission (to M.G.); the Sarnoff Cardiovascular Research Foundation (to A.H.S.); and F.P. Johnson, Jr. (to H.Y.C. and M.T.L.). H.Y.C. is an investigator of the Howard Hughes Medical Institute. Publisher Copyright: {\textcopyright} 2020 The Authors",
year = "2020",
month = nov,
day = "10",
doi = "10.1016/j.celrep.2020.108356",
language = "English (US)",
volume = "33",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "6",
}