Protocol for assessing the role of hippocampal perineuronal nets in aversive memories

Vladimir Jovasevic, Hui Zhang, Zorica Petrovic, Ana Cicvaric, Jelena Radulovic

Research output: Contribution to journalArticlepeer-review


Perineuronal nets (PNNs) are emerging as critical regulators of memory-related neuronal processes. However, their exact contribution depends on type of memory, consolidation stage, or brain region, and remains to be fully investigated. We describe here a protocol to evaluate the importance of PNNs in the dorsal hippocampus in different stages of aversive memories using a mouse model. The protocol provides detailed instructions for surgical implantation of hippocampal cannulas, drug infusion, contextual fear conditioning procedures, and immunohistochemistry for PNN visualization. For complete details on the use and execution of this protocol, please refer to Jovasevic et al. (2021).

Original languageEnglish (US)
Article number100931
JournalSTAR Protocols
Issue number4
StatePublished - Dec 17 2021


  • Behavior
  • Microscopy
  • Model Organisms
  • Neuroscience

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Neuroscience(all)
  • Immunology and Microbiology(all)
  • Medicine(all)


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