TY - JOUR
T1 - Proto-oncogene expression in cloned T lymphocytes
T2 - Mitogens and growth factors induce different patterns of expression
AU - Reed, J. C.
AU - Alpers, J. D.
AU - Scherle, P. A.
AU - Hoover, R. G.
AU - Nowell, P. C.
AU - Prystowsky, M. B.
PY - 1987
Y1 - 1987
N2 - We have compared the effects of a mitogenic lectin, concanavalin A (ConA), and a growth factor, interleukin-2 (IL-2), on the expression of the c-myc, c-fos, and c-myb proto-oncogenes in cloned T lymphocytes. Accumulation of c-myc mRNA was induced by both ConA and IL-2 in these cells. In contrast, expression of c-fos was stimulated primarily by ConA, and accumulation of c-myb mRNA was induced predominantly by IL-2. Thus, ConA and IL-2 induce expression of overlapping, but non-identical, sets of proto-oncogenes in T lymphocytes. Investigations with several different cloned T cells revealed that: (1) c-myb is not induced in all T cells stimulated to grow, indicating that its expression may not be absolutely required for their proliferation; and (2) expression of c-myc, even in combination with c-fos, can be insufficient for growth, demonstrating functional differences between cellular and viral oncogenes in T cells. These observation provide insight into the roles of the c-myc, c-fos, and c-myb proto-oncogenes in normal T cell responses.
AB - We have compared the effects of a mitogenic lectin, concanavalin A (ConA), and a growth factor, interleukin-2 (IL-2), on the expression of the c-myc, c-fos, and c-myb proto-oncogenes in cloned T lymphocytes. Accumulation of c-myc mRNA was induced by both ConA and IL-2 in these cells. In contrast, expression of c-fos was stimulated primarily by ConA, and accumulation of c-myb mRNA was induced predominantly by IL-2. Thus, ConA and IL-2 induce expression of overlapping, but non-identical, sets of proto-oncogenes in T lymphocytes. Investigations with several different cloned T cells revealed that: (1) c-myb is not induced in all T cells stimulated to grow, indicating that its expression may not be absolutely required for their proliferation; and (2) expression of c-myc, even in combination with c-fos, can be insufficient for growth, demonstrating functional differences between cellular and viral oncogenes in T cells. These observation provide insight into the roles of the c-myc, c-fos, and c-myb proto-oncogenes in normal T cell responses.
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M3 - Article
C2 - 3501855
AN - SCOPUS:0023242258
SN - 0950-9232
VL - 1
SP - 223
EP - 228
JO - Oncogene
JF - Oncogene
IS - 2
ER -