Proto-oncogene expression in cloned T lymphocytes

Mitogens and growth factors induce different patterns of expression

J. C. Reed, J. D. Alpers, P. A. Scherle, R. G. Hoover, P. C. Nowell, Michael B. Prystowsky

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

We have compared the effects of a mitogenic lectin, concanavalin A (ConA), and a growth factor, interleukin-2 (IL-2), on the expression of the c-myc, c-fos, and c-myb proto-oncogenes in cloned T lymphocytes. Accumulation of c-myc mRNA was induced by both ConA and IL-2 in these cells. In contrast, expression of c-fos was stimulated primarily by ConA, and accumulation of c-myb mRNA was induced predominantly by IL-2. Thus, ConA and IL-2 induce expression of overlapping, but non-identical, sets of proto-oncogenes in T lymphocytes. Investigations with several different cloned T cells revealed that: (1) c-myb is not induced in all T cells stimulated to grow, indicating that its expression may not be absolutely required for their proliferation; and (2) expression of c-myc, even in combination with c-fos, can be insufficient for growth, demonstrating functional differences between cellular and viral oncogenes in T cells. These observation provide insight into the roles of the c-myc, c-fos, and c-myb proto-oncogenes in normal T cell responses.

Original languageEnglish (US)
Pages (from-to)223-228
Number of pages6
JournalOncogene
Volume1
Issue number2
StatePublished - 1987
Externally publishedYes

Fingerprint

Proto-Oncogenes
Mitogens
Intercellular Signaling Peptides and Proteins
Concanavalin A
T-Lymphocytes
Interleukin-2
myb Genes
Messenger RNA
Oncogenes
Lectins
Observation
Growth

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

Cite this

Reed, J. C., Alpers, J. D., Scherle, P. A., Hoover, R. G., Nowell, P. C., & Prystowsky, M. B. (1987). Proto-oncogene expression in cloned T lymphocytes: Mitogens and growth factors induce different patterns of expression. Oncogene, 1(2), 223-228.

Proto-oncogene expression in cloned T lymphocytes : Mitogens and growth factors induce different patterns of expression. / Reed, J. C.; Alpers, J. D.; Scherle, P. A.; Hoover, R. G.; Nowell, P. C.; Prystowsky, Michael B.

In: Oncogene, Vol. 1, No. 2, 1987, p. 223-228.

Research output: Contribution to journalArticle

Reed, JC, Alpers, JD, Scherle, PA, Hoover, RG, Nowell, PC & Prystowsky, MB 1987, 'Proto-oncogene expression in cloned T lymphocytes: Mitogens and growth factors induce different patterns of expression', Oncogene, vol. 1, no. 2, pp. 223-228.
Reed, J. C. ; Alpers, J. D. ; Scherle, P. A. ; Hoover, R. G. ; Nowell, P. C. ; Prystowsky, Michael B. / Proto-oncogene expression in cloned T lymphocytes : Mitogens and growth factors induce different patterns of expression. In: Oncogene. 1987 ; Vol. 1, No. 2. pp. 223-228.
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