Proteomics and posttranslational protein modifications in Toxoplasma gondii

Louis M. Weiss; Jonathan Wastling; Victoria Jeffers; William J. Sullivan; Kami Kim

doi:10.1016/B978-0-12-815041-2.00022-0

Proteomics and posttranslational protein modifications in Toxoplasma gondii

Louis M. Weiss, Jonathan Wastling, Victoria Jeffers, William J. Sullivan, Kami Kim

Pathology

Research output: Chapter in Book/Report/Conference proceeding › Chapter

2 Scopus citations

Abstract

The identification and characterization of the function of proteins in Toxoplasma gondii has been a focus of many research groups since the development of techniques that enabled the study of individual genes and molecules. Proteomic studies provide complementary data to those obtained by transcriptional “omics” approaches. Proteomics data for T. gondii has also been helpful for improving genome annotation. Improved methods to purify subproteomes and the use of biotin identification (and similar techniques) are dramatically expanding our understanding of the structures formed by this organism and their relationship to its host cell. This chapter reviews the current status of proteomic studies on T. gondii and the role of various posttranslational modifications in the biology of this pathogen.

Original language	English (US)
Title of host publication	Toxoplasma Gondii
Subtitle of host publication	The Model Apicomplexan - Perspectives and Methods
Publisher	Elsevier
Pages	983-1020
Number of pages	38
ISBN (Electronic)	9780128150412
DOIs	https://doi.org/10.1016/B978-0-12-815041-2.00022-0
State	Published - Jan 1 2020

Keywords

Acetylation
BioID
Glycosylation
Mass spectrometry
Methylation
Palmitoylation
Phosphorylation
Posttranslational modification
Protein function
Proteomics
SUMOylation
Succinyllysine
Toxoplasma
Ubiquitination

ASJC Scopus subject areas

General Immunology and Microbiology

Access to Document

10.1016/B978-0-12-815041-2.00022-0

Cite this

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title = "Proteomics and posttranslational protein modifications in Toxoplasma gondii",

abstract = "The identification and characterization of the function of proteins in Toxoplasma gondii has been a focus of many research groups since the development of techniques that enabled the study of individual genes and molecules. Proteomic studies provide complementary data to those obtained by transcriptional “omics” approaches. Proteomics data for T. gondii has also been helpful for improving genome annotation. Improved methods to purify subproteomes and the use of biotin identification (and similar techniques) are dramatically expanding our understanding of the structures formed by this organism and their relationship to its host cell. This chapter reviews the current status of proteomic studies on T. gondii and the role of various posttranslational modifications in the biology of this pathogen.",

keywords = "Acetylation, BioID, Glycosylation, Mass spectrometry, Methylation, Palmitoylation, Phosphorylation, Posttranslational modification, Protein function, Proteomics, SUMOylation, Succinyllysine, Toxoplasma, Ubiquitination",

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AU - Wastling, Jonathan

AU - Jeffers, Victoria

AU - Sullivan, William J.

AU - Kim, Kami

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AB - The identification and characterization of the function of proteins in Toxoplasma gondii has been a focus of many research groups since the development of techniques that enabled the study of individual genes and molecules. Proteomic studies provide complementary data to those obtained by transcriptional “omics” approaches. Proteomics data for T. gondii has also been helpful for improving genome annotation. Improved methods to purify subproteomes and the use of biotin identification (and similar techniques) are dramatically expanding our understanding of the structures formed by this organism and their relationship to its host cell. This chapter reviews the current status of proteomic studies on T. gondii and the role of various posttranslational modifications in the biology of this pathogen.

KW - Acetylation

KW - BioID

KW - Glycosylation

KW - Mass spectrometry

KW - Methylation

KW - Palmitoylation

KW - Phosphorylation

KW - Posttranslational modification

KW - Protein function

KW - Proteomics

KW - SUMOylation

KW - Succinyllysine

KW - Toxoplasma

KW - Ubiquitination

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BT - Toxoplasma Gondii

PB - Elsevier

ER -

Proteomics and posttranslational protein modifications in Toxoplasma gondii

Abstract

Keywords

ASJC Scopus subject areas

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