TY - JOUR
T1 - Proteomic changes during intestinal cell maturation in vivo
AU - Chang, Jinsook
AU - Chance, Mark R.
AU - Nicholas, Courtney
AU - Ahmed, Naseem
AU - Guilmeau, Sandra
AU - Flandez, Marta
AU - Wang, Donghai
AU - Byun, Do Sun
AU - Nasser, Shannon
AU - Albanese, Joseph M.
AU - Corner, Georgia A.
AU - Heerdt, Barbara G.
AU - Wilson, Andrew J.
AU - Augenlicht, Leonard H.
AU - Mariadason, John M.
PY - 2008/12/2
Y1 - 2008/12/2
N2 - Intestinal epithelial cells undergo progressive cell maturation as they migrate along the crypt-villus axis. To determine molecular signatures that define this process, proteins differentially expressed between the crypt and villus were identified by 2D-DIGE and MALDI-MS. Forty-six differentially expressed proteins were identified, several of which were validated by immunohistochemistry. Proteins upregulated in the villus were enriched for those involved in brush border assembly and lipid uptake, established features of differentiated intestinal epithelial cells. Multiple proteins involved in glycolysis were also upregulated in the villus, suggesting increased glycolysis is a feature of intestinal cell differentiation. Conversely, proteins involved in nucleotide metabolism, and protein processing and folding were increased in the crypt, consistent with functions associated with cell proliferation. Three novel paneth cell markers, AGR2, HSPA5 and RRBP1 were also identified. Notably, significant correlation was observed between overall proteomic changes and corresponding gene expression changes along the crypt-villus axis, indicating intestinal cell maturation is primarily regulated at the transcriptional level. This proteomic profiling analysis identified several novel proteins and functional processes differentially induced during intestinal cell maturation in vivo. Integration of proteomic, immunohistochemical, and parallel gene expression datasets demonstrate the coordinated manner in which intestinal cell maturation is regulated.
AB - Intestinal epithelial cells undergo progressive cell maturation as they migrate along the crypt-villus axis. To determine molecular signatures that define this process, proteins differentially expressed between the crypt and villus were identified by 2D-DIGE and MALDI-MS. Forty-six differentially expressed proteins were identified, several of which were validated by immunohistochemistry. Proteins upregulated in the villus were enriched for those involved in brush border assembly and lipid uptake, established features of differentiated intestinal epithelial cells. Multiple proteins involved in glycolysis were also upregulated in the villus, suggesting increased glycolysis is a feature of intestinal cell differentiation. Conversely, proteins involved in nucleotide metabolism, and protein processing and folding were increased in the crypt, consistent with functions associated with cell proliferation. Three novel paneth cell markers, AGR2, HSPA5 and RRBP1 were also identified. Notably, significant correlation was observed between overall proteomic changes and corresponding gene expression changes along the crypt-villus axis, indicating intestinal cell maturation is primarily regulated at the transcriptional level. This proteomic profiling analysis identified several novel proteins and functional processes differentially induced during intestinal cell maturation in vivo. Integration of proteomic, immunohistochemical, and parallel gene expression datasets demonstrate the coordinated manner in which intestinal cell maturation is regulated.
KW - Crypt
KW - Intestine
KW - Proteomics
KW - Villus
UR - http://www.scopus.com/inward/record.url?scp=55849094738&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=55849094738&partnerID=8YFLogxK
U2 - 10.1016/j.jprot.2008.08.003
DO - 10.1016/j.jprot.2008.08.003
M3 - Article
C2 - 18824147
AN - SCOPUS:55849094738
SN - 1874-3919
VL - 71
SP - 530
EP - 546
JO - Journal of Proteomics
JF - Journal of Proteomics
IS - 5
ER -