Proteomic changes during intestinal cell maturation in vivo

Jinsook Chang, Mark R. Chance, Courtney Nicholas, Naseem Ahmed, Sandra Guilmeau, Marta Flandez, Donghai Wang, Do Sun Byun, Shannon Nasser, Joseph M. Albanese, Georgia A. Corner, Barbara G. Heerdt, Andrew J. Wilson, Leonard H. Augenlicht, John M. Mariadason

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Intestinal epithelial cells undergo progressive cell maturation as they migrate along the crypt-villus axis. To determine molecular signatures that define this process, proteins differentially expressed between the crypt and villus were identified by 2D-DIGE and MALDI-MS. Forty-six differentially expressed proteins were identified, several of which were validated by immunohistochemistry. Proteins upregulated in the villus were enriched for those involved in brush border assembly and lipid uptake, established features of differentiated intestinal epithelial cells. Multiple proteins involved in glycolysis were also upregulated in the villus, suggesting increased glycolysis is a feature of intestinal cell differentiation. Conversely, proteins involved in nucleotide metabolism, and protein processing and folding were increased in the crypt, consistent with functions associated with cell proliferation. Three novel paneth cell markers, AGR2, HSPA5 and RRBP1 were also identified. Notably, significant correlation was observed between overall proteomic changes and corresponding gene expression changes along the crypt-villus axis, indicating intestinal cell maturation is primarily regulated at the transcriptional level. This proteomic profiling analysis identified several novel proteins and functional processes differentially induced during intestinal cell maturation in vivo. Integration of proteomic, immunohistochemical, and parallel gene expression datasets demonstrate the coordinated manner in which intestinal cell maturation is regulated.

Original languageEnglish (US)
Pages (from-to)530-546
Number of pages17
JournalJournal of Proteomics
Volume71
Issue number5
DOIs
StatePublished - Dec 2 2008

Fingerprint

Proteomics
Proteins
Glycolysis
Gene expression
Epithelial Cells
Two-Dimensional Difference Gel Electrophoresis
Paneth Cells
Gene Expression
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Protein Folding
Microvilli
Cell proliferation
Brushes
Cell Differentiation
Metabolism
Nucleotides
Immunohistochemistry
Cell Proliferation
Lipids
Processing

Keywords

  • Crypt
  • Intestine
  • Proteomics
  • Villus

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics

Cite this

Chang, J., Chance, M. R., Nicholas, C., Ahmed, N., Guilmeau, S., Flandez, M., ... Mariadason, J. M. (2008). Proteomic changes during intestinal cell maturation in vivo. Journal of Proteomics, 71(5), 530-546. https://doi.org/10.1016/j.jprot.2008.08.003

Proteomic changes during intestinal cell maturation in vivo. / Chang, Jinsook; Chance, Mark R.; Nicholas, Courtney; Ahmed, Naseem; Guilmeau, Sandra; Flandez, Marta; Wang, Donghai; Byun, Do Sun; Nasser, Shannon; Albanese, Joseph M.; Corner, Georgia A.; Heerdt, Barbara G.; Wilson, Andrew J.; Augenlicht, Leonard H.; Mariadason, John M.

In: Journal of Proteomics, Vol. 71, No. 5, 02.12.2008, p. 530-546.

Research output: Contribution to journalArticle

Chang, J, Chance, MR, Nicholas, C, Ahmed, N, Guilmeau, S, Flandez, M, Wang, D, Byun, DS, Nasser, S, Albanese, JM, Corner, GA, Heerdt, BG, Wilson, AJ, Augenlicht, LH & Mariadason, JM 2008, 'Proteomic changes during intestinal cell maturation in vivo', Journal of Proteomics, vol. 71, no. 5, pp. 530-546. https://doi.org/10.1016/j.jprot.2008.08.003
Chang J, Chance MR, Nicholas C, Ahmed N, Guilmeau S, Flandez M et al. Proteomic changes during intestinal cell maturation in vivo. Journal of Proteomics. 2008 Dec 2;71(5):530-546. https://doi.org/10.1016/j.jprot.2008.08.003
Chang, Jinsook ; Chance, Mark R. ; Nicholas, Courtney ; Ahmed, Naseem ; Guilmeau, Sandra ; Flandez, Marta ; Wang, Donghai ; Byun, Do Sun ; Nasser, Shannon ; Albanese, Joseph M. ; Corner, Georgia A. ; Heerdt, Barbara G. ; Wilson, Andrew J. ; Augenlicht, Leonard H. ; Mariadason, John M. / Proteomic changes during intestinal cell maturation in vivo. In: Journal of Proteomics. 2008 ; Vol. 71, No. 5. pp. 530-546.
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