Proteomic analysis reveals novel binding partners of metabotropic glutamate receptor 1

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Regulated trafficking of neurotransmitter receptors is critical to normal neurodevelopment and neuronal signaling. Group I mGluRs (mGluR1/5 and their splice variants) are G protein-coupled receptors enriched at excitatory synapses, where they serve to modulate glutamatergic transmission. The mGluR1 splice variants mGluR1a and mGluR1b are broadly expressed in the central nervous system and differ in their signaling and trafficking properties. Several proteins have been identified that selectively interact with mGluR1a and participate in receptor trafficking but no proteins interacting with mGluR1b have thus far been reported. We have used a proteomic strategy to isolate and identify proteins that co-purify with mGluR1b in Madin-Darby Canine Kidney (MDCK) cells, an established model system for trafficking studies. Here, we report the identification of 10 novel candidate mGluR1b-interacting proteins. Several of the identified proteins are structural components of the cell cytoskeleton, while others serve as cytoskeleton-associated adaptors and motors or endoplasmic reticulum-associated chaperones. Findings from this work will help unravel the complex cellular mechanisms underlying mGluR trafficking under physiological and pathological conditions.

Original languageEnglish (US)
Pages (from-to)1515-1525
Number of pages11
JournalJournal of Neurochemistry
Volume108
Issue number6
DOIs
StatePublished - Mar 1 2009

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Keywords

  • G protein-coupled receptors
  • Interacting proteins
  • MGluR1b
  • Metabotropic glutamate receptors
  • Proteomics
  • Trafficking

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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