Proteomic analysis of polymorphonuclear neutrophils identifies catalase as a novel biomarker of abdominal aortic aneurysm: Potential implication of oxidative stress in abdominal aortic aneurysm progression

Priscila Ramos-Mozo, Julio Madrigal-Matute, Roxana Martinez-Pinna, Luis Miguel Blanco-Colio, Juan Antonio Lopez, Emilio Camafeita, Olivier Meilhac, Jean Baptiste Michel, Cesar Aparicio, Melina Vega De Ceniga, Jesus Egido, José Luis Martín-Ventura

Research output: Contribution to journalArticle

52 Scopus citations


Objective-Polymorphonuclear neutrophils (PMNs) play a main role in abdominal aortic aneurysm (AAA) progression. We have analyzed circulating PMNs isolated from AAA patients and controls by a proteomic approach to identify proteins potentially involved in AAA pathogenesis. Methods and results-PMNs from 8 AAA patients (4 large AAA >5 cm and 4 small AAA 3-5 cm) and 4 controls were analyzed by 2D differential in-gel electrophoresis. Among differentially expressed spots, several proteins involved in redox balance were identified by mass spectrometry (eg, cyclophilin, thioredoxin reductase, catalase). Diminished catalase expression and activity were observed in PMNs from AAA patients compared with controls. In contrast, PMNs from AAA patients displayed higher H 2O 2 and myeloperoxidase levels than PMNs from controls. Moreover, a significant decrease in catalase mRNA levels was observed in PMNs after phorbol 12-myristate 13-acetate incubation. Catalase plasma levels were also decreased in large (n=47) and small (n=56) AAA patients compared with controls (n=34). We observed catalase expression in AAA thrombus and thrombus-conditioned medium, associated with PMN infiltration. Furthermore, increased H 2O 2 levels were observed in AAA thrombus-conditioned medium compared with the media layer. Conclusion-Diminished catalase levels in circulating PMNs and plasma are observed in AAA patients, supporting an important role of oxidative stress in AAA evolution.

Original languageEnglish (US)
Pages (from-to)3011-3019
Number of pages9
JournalArteriosclerosis, thrombosis, and vascular biology
Issue number12
Publication statusPublished - Dec 1 2011



  • aneurysms
  • antioxidants
  • leukocytes

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

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