Proteomic analysis of oral cavity squamous cell carcinoma specimens identifies patient outcome-associated proteins

Thomas M. Harris, Peicheng Du, Nicole Kawachi, Thomas J. Belbin, Yanhua Wang, Nicolas F. Schlecht, Thomas J. Ow, Christian E. Keller, Geoffrey J. Childs, Richard V. Smith, Ruth Hogue Angeletti, Michael B. Prystowsky, Jihyeon Lim

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Context.-Global proteomic analysis of oral cavity squamous cell carcinoma was performed to identify changes that reflect patient outcomes. Objectives.-To identify differentially expressed proteins associated with patient outcomes and to explore the use of imaging mass spectrometry as a clinical tool to identify clinically relevant proteins. Design.-Two-dimensional separation of digested peptides generated from 43 specimens with high-resolution mass spectrometry identified proteins associated with disease-specific death, distant metastasis, and loco-regional recurrence. RNA expressions had been correlated to protein levels to test transcriptional regulation of clinically relevant proteins. Imaging mass spectrometry explored an alternative platform for assessing clinically relevant proteins that would complement surgical pathologic diagnosis. Results.-Seventy-two peptide features were found to be associated with 3 patient outcomes: disease-specific death (9), distant metastasis (16), and loco-regional recurrence (39); 8 of them were associated with multiple outcomes. Functional ontology revealed major changes in cell adhesion and calcium binding. Thirteen RNAs showed strong correlation with their encoded proteins, implying transcriptional control. Reduction of DSP, PKP1, and TRIM29 was associated with significantly shorter time to onset of distant metastasis. Reduction of PKP1 and TRIM29 correlated with poorer disease-specific survival. Additionally, S100A8 and S100A9 reductions were verified for their association with poor prognosis using imaging mass spectrometry, a platform more adaptable for use with surgical pathology. Conclusions.-Using global proteomic analysis, we have identified proteins associated with clinical outcomes. The list of clinically relevant proteins observed will provide a means to develop clinical assays for prognosis and optimizing treatment selection.

Original languageEnglish (US)
Pages (from-to)494-507
Number of pages14
JournalArchives of Pathology and Laboratory Medicine
Volume139
Issue number4
DOIs
StatePublished - Apr 1 2015

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Proteomics
Mouth
Squamous Cell Carcinoma
Mass Spectrometry
Proteins
Neoplasm Metastasis
RNA
Recurrence
Surgical Pathology
Peptides
Cell Adhesion
Complement System Proteins
Calcium
Survival

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Medical Laboratory Technology

Cite this

Proteomic analysis of oral cavity squamous cell carcinoma specimens identifies patient outcome-associated proteins. / Harris, Thomas M.; Du, Peicheng; Kawachi, Nicole; Belbin, Thomas J.; Wang, Yanhua; Schlecht, Nicolas F.; Ow, Thomas J.; Keller, Christian E.; Childs, Geoffrey J.; Smith, Richard V.; Angeletti, Ruth Hogue; Prystowsky, Michael B.; Lim, Jihyeon.

In: Archives of Pathology and Laboratory Medicine, Vol. 139, No. 4, 01.04.2015, p. 494-507.

Research output: Contribution to journalArticle

Harris, Thomas M. ; Du, Peicheng ; Kawachi, Nicole ; Belbin, Thomas J. ; Wang, Yanhua ; Schlecht, Nicolas F. ; Ow, Thomas J. ; Keller, Christian E. ; Childs, Geoffrey J. ; Smith, Richard V. ; Angeletti, Ruth Hogue ; Prystowsky, Michael B. ; Lim, Jihyeon. / Proteomic analysis of oral cavity squamous cell carcinoma specimens identifies patient outcome-associated proteins. In: Archives of Pathology and Laboratory Medicine. 2015 ; Vol. 139, No. 4. pp. 494-507.
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