Proteomic analysis of microglia-derived exosomes

Metabolic role of the aminopeptidase CD13 in neuropeptide catabolism

Ilaria Potolicchio, Gregory J. Carven, Xiaonan Xu, Christopher Stipp, Richiard J. Riese, Lawrence J. Stern, Laura Santambrogio

Research output: Contribution to journalArticle

183 Citations (Scopus)

Abstract

Vesicle transport is a fundamental mechanism of communication in the CNS. In this study we characterized a novel type of vesicle released by murine brain microglial cells: microglial exosomes. Analysis of their protein content identified several enzymes, chaperones, tetraspanins, and membrane receptors previously reported in B cells and dendritic cell-derived exosomes. Additionally, microglia-derived exosomes expressed the aminopeptidase CD13 and the lactate transporter MCT-1. Exosomal CD13 was metabolically active in cleaving leucine- and methionine-enkephalins peptides by releasing the N-terminal tyrosine. Cleaved neuropeptides were unable to bind to the neuronal opioid receptor as assessed by cAMP response. Microglial exosomal vesicles may represent an important, previously unrecognized, cellular communication system in an organ in which cell motility is highly restricted.

Original languageEnglish (US)
Pages (from-to)2237-2243
Number of pages7
JournalJournal of Immunology
Volume175
Issue number4
StatePublished - Aug 15 2005

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Exosomes
Aminopeptidases
Microglia
Neuropeptides
Proteomics
Monocarboxylic Acid Transporters
Tetraspanins
Leucine Enkephalin
Transport Vesicles
Methionine Enkephalin
Opioid Receptors
Dendritic Cells
Cell Movement
Tyrosine
B-Lymphocytes
Peptides
Membranes
Brain
Enzymes
Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Potolicchio, I., Carven, G. J., Xu, X., Stipp, C., Riese, R. J., Stern, L. J., & Santambrogio, L. (2005). Proteomic analysis of microglia-derived exosomes: Metabolic role of the aminopeptidase CD13 in neuropeptide catabolism. Journal of Immunology, 175(4), 2237-2243.

Proteomic analysis of microglia-derived exosomes : Metabolic role of the aminopeptidase CD13 in neuropeptide catabolism. / Potolicchio, Ilaria; Carven, Gregory J.; Xu, Xiaonan; Stipp, Christopher; Riese, Richiard J.; Stern, Lawrence J.; Santambrogio, Laura.

In: Journal of Immunology, Vol. 175, No. 4, 15.08.2005, p. 2237-2243.

Research output: Contribution to journalArticle

Potolicchio, I, Carven, GJ, Xu, X, Stipp, C, Riese, RJ, Stern, LJ & Santambrogio, L 2005, 'Proteomic analysis of microglia-derived exosomes: Metabolic role of the aminopeptidase CD13 in neuropeptide catabolism', Journal of Immunology, vol. 175, no. 4, pp. 2237-2243.
Potolicchio I, Carven GJ, Xu X, Stipp C, Riese RJ, Stern LJ et al. Proteomic analysis of microglia-derived exosomes: Metabolic role of the aminopeptidase CD13 in neuropeptide catabolism. Journal of Immunology. 2005 Aug 15;175(4):2237-2243.
Potolicchio, Ilaria ; Carven, Gregory J. ; Xu, Xiaonan ; Stipp, Christopher ; Riese, Richiard J. ; Stern, Lawrence J. ; Santambrogio, Laura. / Proteomic analysis of microglia-derived exosomes : Metabolic role of the aminopeptidase CD13 in neuropeptide catabolism. In: Journal of Immunology. 2005 ; Vol. 175, No. 4. pp. 2237-2243.
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