Proteolytic processing of neuropeptides

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations

Abstract

Proteolytic cleavage of neuropeptide precursors is a major post-translational modification that is essential for the production of all biologically active peptides. Differential processing of precursors can produce peptides with unique biological activities. Even differences of a single amino acid can cause large changes in biological activity. Therefore, it is important to understand the precise molecular form of the peptide that is produced in a particular cell or tissue. For this, mass spectrometry-based peptidomic approaches are ideal. Unlike older radioimmunoassay-based detection techniques, peptidomics methods can measure the precise form of each peptide and can readily distinguish between longer and shorter forms of the same peptide. In addition, peptidomic methods are not limited to known peptides and can detect hundreds of different peptides in a single experiment. Comparison between two or more groups of samples is possible with quantitative peptidomic methods. The use of quantitative methods allow for differences in levels among tissues, cell types, or between wild-type and mutant animals to be determined. This review describes a method for quantitative peptidomics using isotopic labels based on trimethylammonium butyrate, which can be synthesized in five different isotopic forms, allowing multivariate analysis of five different samples in a single liquid chromatography/mass spectrometry run.

Original languageEnglish (US)
Title of host publicationNeuromethods
PublisherHumana Press Inc.
Pages209-220
Number of pages12
Volume114
DOIs
StatePublished - Nov 8 2015

Publication series

NameNeuromethods
Volume114
ISSN (Print)08932336
ISSN (Electronic)19406045

Keywords

  • Carboxypeptidase
  • Peptidase
  • Peptidomics
  • Prohormone convertase
  • Proprotein convertase
  • Protease
  • Proteomics

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Psychiatry and Mental health

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  • Cite this

    Fricker, L. D. (2015). Proteolytic processing of neuropeptides. In Neuromethods (Vol. 114, pp. 209-220). (Neuromethods; Vol. 114). Humana Press Inc.. https://doi.org/10.1007/7657_2015_87