Protein production from the structural genomics perspective: Achievements and future needs

Steven C. Almo, Scott J. Garforth, Brandan S. Hillerich, James D. Love, Ronald D. Seidel, Stephen K. Burley

Research output: Contribution to journalReview article

24 Scopus citations

Abstract

Despite a multitude of recent technical breakthroughs speeding high-resolution structural analysis of biological macromolecules, production of sufficient quantities of well-behaved, active protein continues to represent the rate-limiting step in many structure determination efforts. These challenges are only amplified when considered in the context of ongoing structural genomics efforts, which are now contending with multi-domain eukaryotic proteins, secreted proteins, and ever-larger macromolecular assemblies. Exciting new developments in eukaryotic expression platforms, including insect and mammalian-based systems, promise enhanced opportunities for structural approaches to some of the most important biological problems. Development and implementation of automated eukaryotic expression techniques promises to significantly improve production of materials for structural, functional, and biomedical research applications.

Original languageEnglish (US)
Pages (from-to)335-344
Number of pages10
JournalCurrent Opinion in Structural Biology
Volume23
Issue number3
DOIs
StatePublished - Jun 1 2013

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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