Protein-ligand interactions of the D-galactose/D-glucose-binding protein as a potential sensing probe of glucose biosensors

Olga V. Stepanenko, Olesya V. Stepanenko, Alexander V. Fonin, Vladislav Verkhusha, Irina M. Kuznetsova, Konstantin K. Turoverov

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

In this paper we have studied peculiarities of protein-ligand interaction under different conditions. We have shown that guanidine hydrochloride (GdnHCI) unfolding-refolding of GGBP in the presence of glucose (Glc) is reversible, but the equilibrium curves of complex refolding-unfolding have been attained only after 10-day incubation of GGBP/Glc in the presence of GdnHCl. This effect has not been revealed at heat-induced GGBP/Glc denaturation. Slow equilibration between the native protein in GGBP/Glc complex and the unfolded state of protein in the GdnHCl presence is connected with increased viscosity of solution at moderate and high GdnHCl concentrations which interferes with diffusion of glucose molecules. Thus, the limiting step of the unfolding-refolding process of the complex GGBP/Glc is the disruption/tuning of the configuration fit between the protein in the native state and the ligand.

Original languageEnglish (US)
Pages (from-to)373-379
Number of pages7
JournalSpectroscopy (New York)
Volume27
Issue number5-6
DOIs
StatePublished - 2012

Fingerprint

Galactose
Biosensors
Carrier Proteins
Ligands
Glucose
Proteins
Denaturation
Guanidine
Tuning
Viscosity
Molecules

ASJC Scopus subject areas

  • Spectroscopy

Cite this

Protein-ligand interactions of the D-galactose/D-glucose-binding protein as a potential sensing probe of glucose biosensors. / Stepanenko, Olga V.; Stepanenko, Olesya V.; Fonin, Alexander V.; Verkhusha, Vladislav; Kuznetsova, Irina M.; Turoverov, Konstantin K.

In: Spectroscopy (New York), Vol. 27, No. 5-6, 2012, p. 373-379.

Research output: Contribution to journalArticle

Stepanenko, Olga V. ; Stepanenko, Olesya V. ; Fonin, Alexander V. ; Verkhusha, Vladislav ; Kuznetsova, Irina M. ; Turoverov, Konstantin K. / Protein-ligand interactions of the D-galactose/D-glucose-binding protein as a potential sensing probe of glucose biosensors. In: Spectroscopy (New York). 2012 ; Vol. 27, No. 5-6. pp. 373-379.
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AU - Kuznetsova, Irina M.

AU - Turoverov, Konstantin K.

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