Protein footprinting by pyrite shrink-wrap laminate

Micheal Leser, Jonathan Pegan, Mohammed El Makkaoui, Joerg C. Schlatterer, Michelle Khine, Matt Law, Michael D. Brenowitz

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The structure of macromolecules and their complexes dictate their biological function. In "footprinting", the solvent accessibility of the residues that constitute proteins, DNA and RNA can be determined from their reactivity to an exogenous reagent such as the hydroxyl radical (·OH). While ·OH generation for protein footprinting is achieved by radiolysis, photolysis and electrochemistry, we present a simpler solution. A thin film of pyrite (cubic FeS2) nanocrystals deposited onto a shape memory polymer (commodity shrink-wrap film) generates sufficient ·OH via Fenton chemistry for oxidative footprinting analysis of proteins. We demonstrate that varying either time or H2O2 concentration yields the required ·OH dose-oxidation response relationship. A simple and scalable sample handling protocol is enabled by thermoforming the "pyrite shrink-wrap laminate" into a standard microtiter plate format. The low cost and malleability of the laminate facilitates its integration into high throughput screening and microfluidic devices.

Original languageEnglish (US)
Pages (from-to)1646-1650
Number of pages5
JournalLab on a Chip - Miniaturisation for Chemistry and Biology
Volume15
Issue number7
DOIs
StatePublished - Apr 7 2015

Fingerprint

Protein Footprinting
Pyrites
Lab-On-A-Chip Devices
Laminates
Proteins
Electrochemistry
Photolysis
Hydroxyl Radical
Nanoparticles
Thermoforming
Radiolysis
Polymers
RNA
Macromolecules
Shape memory effect
Microfluidics
Costs and Cost Analysis
Nanocrystals
DNA
Screening

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)
  • Bioengineering
  • Biomedical Engineering

Cite this

Leser, M., Pegan, J., El Makkaoui, M., Schlatterer, J. C., Khine, M., Law, M., & Brenowitz, M. D. (2015). Protein footprinting by pyrite shrink-wrap laminate. Lab on a Chip - Miniaturisation for Chemistry and Biology, 15(7), 1646-1650. https://doi.org/10.1039/c4lc01288g

Protein footprinting by pyrite shrink-wrap laminate. / Leser, Micheal; Pegan, Jonathan; El Makkaoui, Mohammed; Schlatterer, Joerg C.; Khine, Michelle; Law, Matt; Brenowitz, Michael D.

In: Lab on a Chip - Miniaturisation for Chemistry and Biology, Vol. 15, No. 7, 07.04.2015, p. 1646-1650.

Research output: Contribution to journalArticle

Leser, M, Pegan, J, El Makkaoui, M, Schlatterer, JC, Khine, M, Law, M & Brenowitz, MD 2015, 'Protein footprinting by pyrite shrink-wrap laminate', Lab on a Chip - Miniaturisation for Chemistry and Biology, vol. 15, no. 7, pp. 1646-1650. https://doi.org/10.1039/c4lc01288g
Leser M, Pegan J, El Makkaoui M, Schlatterer JC, Khine M, Law M et al. Protein footprinting by pyrite shrink-wrap laminate. Lab on a Chip - Miniaturisation for Chemistry and Biology. 2015 Apr 7;15(7):1646-1650. https://doi.org/10.1039/c4lc01288g
Leser, Micheal ; Pegan, Jonathan ; El Makkaoui, Mohammed ; Schlatterer, Joerg C. ; Khine, Michelle ; Law, Matt ; Brenowitz, Michael D. / Protein footprinting by pyrite shrink-wrap laminate. In: Lab on a Chip - Miniaturisation for Chemistry and Biology. 2015 ; Vol. 15, No. 7. pp. 1646-1650.
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