Protein folding following synthesis in vitro and in vivo: Association of newly synthesized protein with 50S subunit of E. coli ribosome

Arunima Basu; Dibyendu Samanta; Arpita Bhattacharya; Anindita Das; Debasis Das; Chanchal DasGupta

doi:10.1016/j.bbrc.2007.11.142

Protein folding following synthesis in vitro and in vivo: Association of newly synthesized protein with 50S subunit of E. coli ribosome

Arunima Basu, Dibyendu Samanta, Arpita Bhattacharya, Anindita Das, Debasis Das, Chanchal DasGupta

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

In the accompanying paper, it was shown that a protein, while reverting to native form from the unfolded state in vitro with the help of bacterial 70S ribosome, split the latter into its subunits (50S and 30S) and remains associated with the 50S subunit. Here, we follow the fate of nascent proteins both in case of in vivo and in vitro translation system. The newly synthesised protein was found to associate with the 50S subunit in both the cases.

Original language	English (US)
Pages (from-to)	592-597
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	366
Issue number	2
DOIs	https://doi.org/10.1016/j.bbrc.2007.11.142
State	Published - Feb 8 2008
Externally published	Yes

Keywords

Association of nascent protein with 50S
Domain V of 23S rRNA
Protein folding
Ribosome assisted protein folding

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2007.11.142

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title = "Protein folding following synthesis in vitro and in vivo: Association of newly synthesized protein with 50S subunit of E. coli ribosome",

abstract = "In the accompanying paper, it was shown that a protein, while reverting to native form from the unfolded state in vitro with the help of bacterial 70S ribosome, split the latter into its subunits (50S and 30S) and remains associated with the 50S subunit. Here, we follow the fate of nascent proteins both in case of in vivo and in vitro translation system. The newly synthesised protein was found to associate with the 50S subunit in both the cases.",

keywords = "Association of nascent protein with 50S, Domain V of 23S rRNA, Protein folding, Ribosome assisted protein folding",

author = "Arunima Basu and Dibyendu Samanta and Arpita Bhattacharya and Anindita Das and Debasis Das and Chanchal DasGupta",

note = "Funding Information: The consistent help from Prof. D.K. Chattoraj, NIH, USA at various stages of the work is gratefully acknowledged. The research was supported by grants from DBT, CSIR, FIST, and UGC-DSA. D.S., A.D., and A.B. are CSIR research fellow.",

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doi = "10.1016/j.bbrc.2007.11.142",

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pages = "592--597",

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T1 - Protein folding following synthesis in vitro and in vivo

T2 - Association of newly synthesized protein with 50S subunit of E. coli ribosome

AU - Basu, Arunima

AU - Samanta, Dibyendu

AU - Bhattacharya, Arpita

AU - Das, Anindita

AU - Das, Debasis

AU - DasGupta, Chanchal

N1 - Funding Information: The consistent help from Prof. D.K. Chattoraj, NIH, USA at various stages of the work is gratefully acknowledged. The research was supported by grants from DBT, CSIR, FIST, and UGC-DSA. D.S., A.D., and A.B. are CSIR research fellow.

PY - 2008/2/8

Y1 - 2008/2/8

N2 - In the accompanying paper, it was shown that a protein, while reverting to native form from the unfolded state in vitro with the help of bacterial 70S ribosome, split the latter into its subunits (50S and 30S) and remains associated with the 50S subunit. Here, we follow the fate of nascent proteins both in case of in vivo and in vitro translation system. The newly synthesised protein was found to associate with the 50S subunit in both the cases.

AB - In the accompanying paper, it was shown that a protein, while reverting to native form from the unfolded state in vitro with the help of bacterial 70S ribosome, split the latter into its subunits (50S and 30S) and remains associated with the 50S subunit. Here, we follow the fate of nascent proteins both in case of in vivo and in vitro translation system. The newly synthesised protein was found to associate with the 50S subunit in both the cases.

KW - Association of nascent protein with 50S

KW - Domain V of 23S rRNA

KW - Protein folding

KW - Ribosome assisted protein folding

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Protein folding following synthesis in vitro and in vivo: Association of newly synthesized protein with 50S subunit of E. coli ribosome

Abstract

Keywords

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