Protein-binding A + T-rich motifs flank the duck βA-globin enhancer

Aleš Cvekl, Květa Horská, Čestmír Vlček, Václav P`ačes

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

The duck βA-globin (βGLB) enhancer DNA was analysed by footprinting for sites of specific binding of proteins extracted from duck erythrocytes. The results were compared with previously determined protein binding to the homologous region in chicken DNA. Two A + T-rich protein-binding sites, not recognized in chicken, were found at the 5'-end and the 3'-end of the duck βGLB enhancer. The 5'-motif (designated BS-1; 5'-AAACAAAATGAA) binds proteins extracted from both embryonic and adult erythrocytes, while the 3 '-motif (55-2 ; 5'-ATAAACAAGGTC) binds protein from embryonic cells only.

Original languageEnglish (US)
Pages (from-to)253-257
Number of pages5
JournalGene
Volume103
Issue number2
DOIs
StatePublished - Jul 22 1991
Externally publishedYes

Keywords

  • Transcription factors
  • avian globin-encoding genes
  • footprinting
  • recombinant DNA

ASJC Scopus subject areas

  • Genetics

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