TY - JOUR
T1 - Protective efficacy of probiotic alone or in conjunction with a prebiotic in Salmonella-induced liver damage
AU - Rishi, Praveen
AU - Mavi, Swapandeep Kaur
AU - Bharrhan, Sushma
AU - Shukla, Geeta
AU - Tewari, Rupinder
PY - 2009/8
Y1 - 2009/8
N2 - In view of the increasing interest in the bioecological and nutritional control of diseases, use of probiotics alone or in combination with prebiotics (synbiotics) appears as a therapeutic option for various diseases. In this study, an attempt was made to explore the protective potential of Lactobacillus acidophilus as a probiotic, inulin as a prebiotic and both L. acidophilus and inulin as synbiotic against Salmonella-induced liver damage in a murine model. The probiotic, prebiotic and synbiotic supplementation resulted in decreased bacterial translocation in the liver of mice challenged with Salmonella typhimurium and decreased levels of serum aminotransferases, suggesting their protective role against Salmonella infection. Mice supplemented with these preparations before Salmonella challenge also revealed decreased levels of lipid peroxidation, increased levels of superoxide dismutase and glutathione, along with reduced levels of nitric oxide. Thus, bacteriological and biochemical alterations correlated well with the histological evidence. Protection afforded by supplementation with the probiotic alone was found to be more effective. None of the observations was suggestive of the synergistic effect in the synbiotic-supplemented animals. Thus, it is indicated that the probiotic and the prebiotic used in the present study may act by different mechanisms involved in affording protection against Salmonella-induced liver damage.
AB - In view of the increasing interest in the bioecological and nutritional control of diseases, use of probiotics alone or in combination with prebiotics (synbiotics) appears as a therapeutic option for various diseases. In this study, an attempt was made to explore the protective potential of Lactobacillus acidophilus as a probiotic, inulin as a prebiotic and both L. acidophilus and inulin as synbiotic against Salmonella-induced liver damage in a murine model. The probiotic, prebiotic and synbiotic supplementation resulted in decreased bacterial translocation in the liver of mice challenged with Salmonella typhimurium and decreased levels of serum aminotransferases, suggesting their protective role against Salmonella infection. Mice supplemented with these preparations before Salmonella challenge also revealed decreased levels of lipid peroxidation, increased levels of superoxide dismutase and glutathione, along with reduced levels of nitric oxide. Thus, bacteriological and biochemical alterations correlated well with the histological evidence. Protection afforded by supplementation with the probiotic alone was found to be more effective. None of the observations was suggestive of the synergistic effect in the synbiotic-supplemented animals. Thus, it is indicated that the probiotic and the prebiotic used in the present study may act by different mechanisms involved in affording protection against Salmonella-induced liver damage.
KW - Lactobacillus acidophilus
KW - Liver damage
KW - Probiotic
KW - Salmonella
UR - http://www.scopus.com/inward/record.url?scp=67650475363&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=67650475363&partnerID=8YFLogxK
U2 - 10.1111/j.1574-6941.2009.00703.x
DO - 10.1111/j.1574-6941.2009.00703.x
M3 - Article
C2 - 19496820
AN - SCOPUS:67650475363
SN - 0168-6496
VL - 69
SP - 222
EP - 230
JO - FEMS Microbiology Ecology
JF - FEMS Microbiology Ecology
IS - 2
ER -