Protective effects of thioredoxin-mediated p53 activation in response to mild hyperthermia

Hwa Jin Jung, Young Rok Seo

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Recently, mild hyperthermia was shown to induce cell cycle arrest at the G 2/M phase transition without leading to DNA damage. The mechanism of this regulation has not yet been elucidated, although p53 has been shown to be activated in response to mild hyperthermia. Here, we report the role of thioredoxin (TXN) in mild hyperthermia-induced cellular responses. Our data showed that the protein levels of p53 and its downstream gene, Gadd45a, which is an indicator of G2/M arrest, were significantly decreased in TXN siRNA-treated cells under conditions of mild hyperthermia (41°C, 60 min) as compared to TXN wild-type cells, implying that TXN might play an important role in mild hyperthermia-induced G 2/M arrest via p53 and Gadd45a activation. Furthermore, the release of cyclin-dependent kinase Cdc2, known to be regulated by Gadd45a under G 2/M arrest, was inhibited from the nucleus for arrest in the G 2/M phase in TXN downregulated cells under mild hyperthermia. We suggest that G 2/M arrest mediated via the TXN-modulated p53 in response to mild hyperthermia may provide critical insight into the clinical use of mild hyperthermia to induce an adaptive response against genotoxic stresses.

Original languageEnglish (US)
Pages (from-to)650-656
Number of pages7
JournalOncology Reports
Volume27
Issue number3
DOIs
StatePublished - Mar 2012
Externally publishedYes

Fingerprint

Thioredoxins
Fever
Induced Hyperthermia
Cell Division
DNA Damage
Cyclin-Dependent Kinases
Phase Transition
Cell Cycle Checkpoints
Small Interfering RNA
Down-Regulation
Genes
Proteins

Keywords

  • Cdc2
  • G /M phase
  • Gadd45a
  • Mild hyperthermia
  • p53
  • Thioredoxin

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Protective effects of thioredoxin-mediated p53 activation in response to mild hyperthermia. / Jung, Hwa Jin; Seo, Young Rok.

In: Oncology Reports, Vol. 27, No. 3, 03.2012, p. 650-656.

Research output: Contribution to journalArticle

Jung, Hwa Jin ; Seo, Young Rok. / Protective effects of thioredoxin-mediated p53 activation in response to mild hyperthermia. In: Oncology Reports. 2012 ; Vol. 27, No. 3. pp. 650-656.
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