TY - JOUR
T1 - Protective Effects of Green Tea Polyphenols in the 6-OHDA Rat Model of Parkinson's Disease Through Inhibition of ROS-NO Pathway
AU - Guo, Shuhong
AU - Yan, Jingqi
AU - Yang, Tangbin
AU - Yang, Xianqiang
AU - Bezard, Erwan
AU - Zhao, Baolu
N1 - Funding Information:
This study was supported by the National Natural Science Foundation of China (Grant No. 30170239) and 973 (Grant No. 2006CB500700), the E-Institutes of Shanghai Municipal Education Commission (Project No. E-04010). University Victor Segalen and the National Center for Scientific Research (The Centre National de la Recherche Scientifique) also provided funding. We thank S. Dovero for technical assistance.
PY - 2007/12/15
Y1 - 2007/12/15
N2 - Background: Nitric oxide (NO) and related pathways are thought to play an important role in the pathogenesis of Parkinson's disease (PD). Our in vitro experiments suggested that green tea polyphenols (GTP) might protect dopamine neurons through inhibition of NO and reactive oxygen species (ROS). Methods: Immunohistochemistry, terminal deoxynucleotidyl transferase-mediated dUTP Nick End Labeling assay, electron spin resonance spin trapping, enzyme linked immunosorbent assay, and molecular biological methods were used to investigate the effects of GTP in an unilateral 6-hydroxydopamine (6-OHDA)-treated rat model of PD. Results: GTP treatment dose-dependently protected dopaminergic neurons by preventing from midbrain and striatal 6-OHDA-induced increase in 1) both ROS and NO levels, 2) lipid peroxidation, 3) nitrite/nitrate content, 4) inducible nitric oxide synthase, and 5) protein-bound 3-nitro-tyrosine. Moreover, GTP treatment dose-dependently preserved the free radical scavenging capability of both the midbrain and the striatum. Conclusions: These results support the in vivo protection of GTP against 6-OHDA and suggest that GTP treatment might represent a neuroprotective treatment of PD.
AB - Background: Nitric oxide (NO) and related pathways are thought to play an important role in the pathogenesis of Parkinson's disease (PD). Our in vitro experiments suggested that green tea polyphenols (GTP) might protect dopamine neurons through inhibition of NO and reactive oxygen species (ROS). Methods: Immunohistochemistry, terminal deoxynucleotidyl transferase-mediated dUTP Nick End Labeling assay, electron spin resonance spin trapping, enzyme linked immunosorbent assay, and molecular biological methods were used to investigate the effects of GTP in an unilateral 6-hydroxydopamine (6-OHDA)-treated rat model of PD. Results: GTP treatment dose-dependently protected dopaminergic neurons by preventing from midbrain and striatal 6-OHDA-induced increase in 1) both ROS and NO levels, 2) lipid peroxidation, 3) nitrite/nitrate content, 4) inducible nitric oxide synthase, and 5) protein-bound 3-nitro-tyrosine. Moreover, GTP treatment dose-dependently preserved the free radical scavenging capability of both the midbrain and the striatum. Conclusions: These results support the in vivo protection of GTP against 6-OHDA and suggest that GTP treatment might represent a neuroprotective treatment of PD.
KW - Electron spin resonance
KW - natural antioxidants
KW - neurodegenerative disease
KW - nitric oxide
KW - reactive oxygen species
KW - stereology
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U2 - 10.1016/j.biopsych.2007.04.020
DO - 10.1016/j.biopsych.2007.04.020
M3 - Article
C2 - 17624318
AN - SCOPUS:36549060678
SN - 0006-3223
VL - 62
SP - 1353
EP - 1362
JO - Biological Psychiatry
JF - Biological Psychiatry
IS - 12
ER -