Protective Effect of Borage Seed Oil and Gamma Linolenic Acid on DNA: In Vivo and In Vitro Studies

Inmaculada Tasset, Zahira Fernández-Bedmar, María Dolores Lozano-Baena, Juan Campos-Sánchez, Antonio de Haro-Bailón, Andrés Muñoz-Serrano, Ángeles Alonso-Moraga

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Borage (Borago officinalis L.) seed oil has been used as a treatment for various degenerative diseases. Many useful properties of this oil are attributed to its high gamma linolenic acid content (GLA, 18:3 ω-6). The purpose of this study was to demonstrate the safety and suitability of the use of borage seed oil, along with one of its active components, GLA, with respect to DNA integrity, and to establish possible in vivo toxic and in vitro cytotoxic effects. In order to measure these properties, five types of assays were carried out: toxicity, genotoxicity, antigenotoxicity, cytotoxicity (using the promyelocytic leukaemia HL60 cell line), and life span (in vivo analysis using the Drosophila model). Results showed that i) Borage seed oil is not toxic to D. melanogaster at physiological concentrations below 125 μl/ml and the studies on GLA indicated non-toxicity at the lowest concentration analyzed ii) Borage seed oil and GLA are DNA safe (non-genotoxic) and antimutagenic compared to hydrogen peroxide, thereby confirming its antioxidant capacity; iii) Borage seed oil and GLA exhibited cytotoxic activity in low doses (IC50 of 1 μl/ml and 0.087 mM, respectively) iv) Low doses of borage seed oil (0.19%) increased the health span of D. melanogaster; and v) GLA significantly decreased the life span of D. melanogaster. Based on the antimutagenic and cytotoxic effects along with the ability to increase the health span, we propose supplementation with borage seed oil rather than GLA, because it protects DNA by modulating oxidative genetic damage in D. melanogaster, increases the health span and exerts cytotoxic activity towards promyelocytic HL60 cells.

Original languageEnglish (US)
Article numbere56986
JournalPLoS One
Volume8
Issue number2
DOIs
StatePublished - Feb 27 2013
Externally publishedYes

Fingerprint

borage oil
gamma-Linolenic Acid
Oilseeds
gamma-linolenic acid
seed oils
in vitro studies
protective effect
Seeds
DNA
Melanogaster
Borago
Borago officinalis
cytotoxicity
Poisons
HL-60 Cells
Health
Oils
Antimutagenic Agents
antimutagenic activity
genotoxicity

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Tasset, I., Fernández-Bedmar, Z., Lozano-Baena, M. D., Campos-Sánchez, J., de Haro-Bailón, A., Muñoz-Serrano, A., & Alonso-Moraga, Á. (2013). Protective Effect of Borage Seed Oil and Gamma Linolenic Acid on DNA: In Vivo and In Vitro Studies. PLoS One, 8(2), [e56986]. https://doi.org/10.1371/journal.pone.0056986

Protective Effect of Borage Seed Oil and Gamma Linolenic Acid on DNA : In Vivo and In Vitro Studies. / Tasset, Inmaculada; Fernández-Bedmar, Zahira; Lozano-Baena, María Dolores; Campos-Sánchez, Juan; de Haro-Bailón, Antonio; Muñoz-Serrano, Andrés; Alonso-Moraga, Ángeles.

In: PLoS One, Vol. 8, No. 2, e56986, 27.02.2013.

Research output: Contribution to journalArticle

Tasset, I, Fernández-Bedmar, Z, Lozano-Baena, MD, Campos-Sánchez, J, de Haro-Bailón, A, Muñoz-Serrano, A & Alonso-Moraga, Á 2013, 'Protective Effect of Borage Seed Oil and Gamma Linolenic Acid on DNA: In Vivo and In Vitro Studies', PLoS One, vol. 8, no. 2, e56986. https://doi.org/10.1371/journal.pone.0056986
Tasset I, Fernández-Bedmar Z, Lozano-Baena MD, Campos-Sánchez J, de Haro-Bailón A, Muñoz-Serrano A et al. Protective Effect of Borage Seed Oil and Gamma Linolenic Acid on DNA: In Vivo and In Vitro Studies. PLoS One. 2013 Feb 27;8(2). e56986. https://doi.org/10.1371/journal.pone.0056986
Tasset, Inmaculada ; Fernández-Bedmar, Zahira ; Lozano-Baena, María Dolores ; Campos-Sánchez, Juan ; de Haro-Bailón, Antonio ; Muñoz-Serrano, Andrés ; Alonso-Moraga, Ángeles. / Protective Effect of Borage Seed Oil and Gamma Linolenic Acid on DNA : In Vivo and In Vitro Studies. In: PLoS One. 2013 ; Vol. 8, No. 2.
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abstract = "Borage (Borago officinalis L.) seed oil has been used as a treatment for various degenerative diseases. Many useful properties of this oil are attributed to its high gamma linolenic acid content (GLA, 18:3 ω-6). The purpose of this study was to demonstrate the safety and suitability of the use of borage seed oil, along with one of its active components, GLA, with respect to DNA integrity, and to establish possible in vivo toxic and in vitro cytotoxic effects. In order to measure these properties, five types of assays were carried out: toxicity, genotoxicity, antigenotoxicity, cytotoxicity (using the promyelocytic leukaemia HL60 cell line), and life span (in vivo analysis using the Drosophila model). Results showed that i) Borage seed oil is not toxic to D. melanogaster at physiological concentrations below 125 μl/ml and the studies on GLA indicated non-toxicity at the lowest concentration analyzed ii) Borage seed oil and GLA are DNA safe (non-genotoxic) and antimutagenic compared to hydrogen peroxide, thereby confirming its antioxidant capacity; iii) Borage seed oil and GLA exhibited cytotoxic activity in low doses (IC50 of 1 μl/ml and 0.087 mM, respectively) iv) Low doses of borage seed oil (0.19{\%}) increased the health span of D. melanogaster; and v) GLA significantly decreased the life span of D. melanogaster. Based on the antimutagenic and cytotoxic effects along with the ability to increase the health span, we propose supplementation with borage seed oil rather than GLA, because it protects DNA by modulating oxidative genetic damage in D. melanogaster, increases the health span and exerts cytotoxic activity towards promyelocytic HL60 cells.",
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