Protection Elicited by a Double Leucine and Pantothenate Auxotroph of Mycobacterium tuberculosis in Guinea Pigs

Samantha L. Sampson, Christopher C. Dascher, Vasan K. Sambandamurthy, Robert G. Russell, William R. Jacobs, Barry R. Bloom, Mary K. Hondalus

Research output: Contribution to journalArticle

89 Citations (Scopus)

Abstract

We developed a live, fully attenuated Mycobacterium tuberculosis vaccine candidate strain with two independent attenuating auxotrophic mutations in leucine and pantothenate biosynthesis. The ΔleuD ΔpanCD double auxotroph is fully attenuated in the SCID mouse model and highly immunogenic and protective in the extremely sensitive guinea pig tuberculosis model, reducing both bacterial burden and disease pathology.

Original languageEnglish (US)
Pages (from-to)3031-3037
Number of pages7
JournalInfection and Immunity
Volume72
Issue number5
DOIs
StatePublished - May 1 2004

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Tuberculosis Vaccines
SCID Mice
Mycobacterium tuberculosis
Leucine
Guinea Pigs
Tuberculosis
Pathology
Mutation

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

Sampson, S. L., Dascher, C. C., Sambandamurthy, V. K., Russell, R. G., Jacobs, W. R., Bloom, B. R., & Hondalus, M. K. (2004). Protection Elicited by a Double Leucine and Pantothenate Auxotroph of Mycobacterium tuberculosis in Guinea Pigs. Infection and Immunity, 72(5), 3031-3037. https://doi.org/10.1128/IAI.72.5.3031-3037.2004

Protection Elicited by a Double Leucine and Pantothenate Auxotroph of Mycobacterium tuberculosis in Guinea Pigs. / Sampson, Samantha L.; Dascher, Christopher C.; Sambandamurthy, Vasan K.; Russell, Robert G.; Jacobs, William R.; Bloom, Barry R.; Hondalus, Mary K.

In: Infection and Immunity, Vol. 72, No. 5, 01.05.2004, p. 3031-3037.

Research output: Contribution to journalArticle

Sampson, Samantha L. ; Dascher, Christopher C. ; Sambandamurthy, Vasan K. ; Russell, Robert G. ; Jacobs, William R. ; Bloom, Barry R. ; Hondalus, Mary K. / Protection Elicited by a Double Leucine and Pantothenate Auxotroph of Mycobacterium tuberculosis in Guinea Pigs. In: Infection and Immunity. 2004 ; Vol. 72, No. 5. pp. 3031-3037.
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