Protection Elicited by a Double Leucine and Pantothenate Auxotroph of Mycobacterium tuberculosis in Guinea Pigs

Samantha L. Sampson, Christopher C. Dascher, Vasan K. Sambandamurthy, Robert G. Russell, William R. Jacobs, Barry R. Bloom, Mary K. Hondalus

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Abstract

We developed a live, fully attenuated Mycobacterium tuberculosis vaccine candidate strain with two independent attenuating auxotrophic mutations in leucine and pantothenate biosynthesis. The ΔleuD ΔpanCD double auxotroph is fully attenuated in the SCID mouse model and highly immunogenic and protective in the extremely sensitive guinea pig tuberculosis model, reducing both bacterial burden and disease pathology.

Original languageEnglish (US)
Pages (from-to)3031-3037
Number of pages7
JournalInfection and Immunity
Volume72
Issue number5
DOIs
Publication statusPublished - May 1 2004

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ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

Sampson, S. L., Dascher, C. C., Sambandamurthy, V. K., Russell, R. G., Jacobs, W. R., Bloom, B. R., & Hondalus, M. K. (2004). Protection Elicited by a Double Leucine and Pantothenate Auxotroph of Mycobacterium tuberculosis in Guinea Pigs. Infection and Immunity, 72(5), 3031-3037. https://doi.org/10.1128/IAI.72.5.3031-3037.2004