Protected nucleotide sequences in nuclear ribonucleoprotein

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

e rapidly labeled nuclear ribonucleic acid in human carcinoma cells which is protected by protein from digestion by staphylococcal nuclease (EC 3.1.4.7) has been investigated. A simple and discrete sequence specificity was not found, but the protected RNA fragments are rich in G + C and were shown by fingerprinting to comprise a nonrandom subset of all heterogeneous nuclear ribonucleic acid (hnRNA) sequences enriched in the sequences AGC, GGC, AGGC, and GAGC. There was no detectable enrichment for double-stranded RNA in the protected fraction. These data provide the first evidence that the association of any protein with hnRNA is nonrandom with respect to nucleotide sequence.

Original languageEnglish (US)
Pages (from-to)3780-3786
Number of pages7
JournalBiochemistry
Volume18
Issue number17
StatePublished - 1979
Externally publishedYes

Fingerprint

Ribonucleoproteins
Nucleotides
RNA
Micrococcal Nuclease
Double-Stranded RNA
Proteolysis
Proteins
Cells
Carcinoma

ASJC Scopus subject areas

  • Biochemistry

Cite this

Protected nucleotide sequences in nuclear ribonucleoprotein. / Augenlicht, Leonard H.

In: Biochemistry, Vol. 18, No. 17, 1979, p. 3780-3786.

Research output: Contribution to journalArticle

@article{01850d2c606a4455a62ac1c76ee5e66b,
title = "Protected nucleotide sequences in nuclear ribonucleoprotein",
abstract = "e rapidly labeled nuclear ribonucleic acid in human carcinoma cells which is protected by protein from digestion by staphylococcal nuclease (EC 3.1.4.7) has been investigated. A simple and discrete sequence specificity was not found, but the protected RNA fragments are rich in G + C and were shown by fingerprinting to comprise a nonrandom subset of all heterogeneous nuclear ribonucleic acid (hnRNA) sequences enriched in the sequences AGC, GGC, AGGC, and GAGC. There was no detectable enrichment for double-stranded RNA in the protected fraction. These data provide the first evidence that the association of any protein with hnRNA is nonrandom with respect to nucleotide sequence.",
author = "Augenlicht, {Leonard H.}",
year = "1979",
language = "English (US)",
volume = "18",
pages = "3780--3786",
journal = "Biochemistry",
issn = "0006-2960",
publisher = "American Chemical Society",
number = "17",

}

TY - JOUR

T1 - Protected nucleotide sequences in nuclear ribonucleoprotein

AU - Augenlicht, Leonard H.

PY - 1979

Y1 - 1979

N2 - e rapidly labeled nuclear ribonucleic acid in human carcinoma cells which is protected by protein from digestion by staphylococcal nuclease (EC 3.1.4.7) has been investigated. A simple and discrete sequence specificity was not found, but the protected RNA fragments are rich in G + C and were shown by fingerprinting to comprise a nonrandom subset of all heterogeneous nuclear ribonucleic acid (hnRNA) sequences enriched in the sequences AGC, GGC, AGGC, and GAGC. There was no detectable enrichment for double-stranded RNA in the protected fraction. These data provide the first evidence that the association of any protein with hnRNA is nonrandom with respect to nucleotide sequence.

AB - e rapidly labeled nuclear ribonucleic acid in human carcinoma cells which is protected by protein from digestion by staphylococcal nuclease (EC 3.1.4.7) has been investigated. A simple and discrete sequence specificity was not found, but the protected RNA fragments are rich in G + C and were shown by fingerprinting to comprise a nonrandom subset of all heterogeneous nuclear ribonucleic acid (hnRNA) sequences enriched in the sequences AGC, GGC, AGGC, and GAGC. There was no detectable enrichment for double-stranded RNA in the protected fraction. These data provide the first evidence that the association of any protein with hnRNA is nonrandom with respect to nucleotide sequence.

UR - http://www.scopus.com/inward/record.url?scp=0018798007&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018798007&partnerID=8YFLogxK

M3 - Article

VL - 18

SP - 3780

EP - 3786

JO - Biochemistry

JF - Biochemistry

SN - 0006-2960

IS - 17

ER -