Prostaglandin transporter PGT is expressed in cell types that synthesize and release prostanoids

Yi Bao, Michael L. Pucci, Brenda S. Chan, Run Lu, Shigekazu Ito, Victor L. Schuster

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

PGT is a broadly expressed transporter of prostaglandins (PGs) and thromboxane that is energetically poised to take up prostanoids across the plasma membrane. To gain insight into the function of PGT, we generated mouse monoclonal antibody 20 against a portion of putative extracellular loop 5 of rat PGT. Immunoblots of endogenous PGT in rat kidney revealed a 65-kDa protein in a zonal pattern corresponding to PG synthesis rates (papilla ≅ medulla > cortex). Immunocytochemically, PGT in rat kidneys was expressed in glomerular endothelial and mesangial cells, arteriolar endothelial and muscularis cells, principal cells of the collecting duct, medullary interstitial cells, medullary vasa rectae endothelia, and papillary surface epithelium. Proximal tubules, which are known to take up and metabolize PGs, were negative. Immunoblotting and immunocytochemistry revealed that rat platelets also express abundant PGT. Coexpression of the PG synthesis apparatus (cyclooxygenase) and PGT by the same cell suggests that prostanoids may undergo release and reuptake.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Renal Physiology
Volume282
Issue number6 51-6
StatePublished - 2002

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Prostaglandins
Endothelial Cells
Kidney
Mesangial Cells
Thromboxanes
Prostaglandin-Endoperoxide Synthases
Immunoblotting
Endothelium
Blood Platelets
Epithelium
Immunohistochemistry
Monoclonal Antibodies
Cell Membrane
Proteins

Keywords

  • Biological transport
  • Carrier proteins
  • Molecular cloning

ASJC Scopus subject areas

  • Physiology

Cite this

Prostaglandin transporter PGT is expressed in cell types that synthesize and release prostanoids. / Bao, Yi; Pucci, Michael L.; Chan, Brenda S.; Lu, Run; Ito, Shigekazu; Schuster, Victor L.

In: American Journal of Physiology - Renal Physiology, Vol. 282, No. 6 51-6, 2002.

Research output: Contribution to journalArticle

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AU - Pucci, Michael L.

AU - Chan, Brenda S.

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AU - Ito, Shigekazu

AU - Schuster, Victor L.

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N2 - PGT is a broadly expressed transporter of prostaglandins (PGs) and thromboxane that is energetically poised to take up prostanoids across the plasma membrane. To gain insight into the function of PGT, we generated mouse monoclonal antibody 20 against a portion of putative extracellular loop 5 of rat PGT. Immunoblots of endogenous PGT in rat kidney revealed a 65-kDa protein in a zonal pattern corresponding to PG synthesis rates (papilla ≅ medulla > cortex). Immunocytochemically, PGT in rat kidneys was expressed in glomerular endothelial and mesangial cells, arteriolar endothelial and muscularis cells, principal cells of the collecting duct, medullary interstitial cells, medullary vasa rectae endothelia, and papillary surface epithelium. Proximal tubules, which are known to take up and metabolize PGs, were negative. Immunoblotting and immunocytochemistry revealed that rat platelets also express abundant PGT. Coexpression of the PG synthesis apparatus (cyclooxygenase) and PGT by the same cell suggests that prostanoids may undergo release and reuptake.

AB - PGT is a broadly expressed transporter of prostaglandins (PGs) and thromboxane that is energetically poised to take up prostanoids across the plasma membrane. To gain insight into the function of PGT, we generated mouse monoclonal antibody 20 against a portion of putative extracellular loop 5 of rat PGT. Immunoblots of endogenous PGT in rat kidney revealed a 65-kDa protein in a zonal pattern corresponding to PG synthesis rates (papilla ≅ medulla > cortex). Immunocytochemically, PGT in rat kidneys was expressed in glomerular endothelial and mesangial cells, arteriolar endothelial and muscularis cells, principal cells of the collecting duct, medullary interstitial cells, medullary vasa rectae endothelia, and papillary surface epithelium. Proximal tubules, which are known to take up and metabolize PGs, were negative. Immunoblotting and immunocytochemistry revealed that rat platelets also express abundant PGT. Coexpression of the PG synthesis apparatus (cyclooxygenase) and PGT by the same cell suggests that prostanoids may undergo release and reuptake.

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