Promoter competition assay for analyzing gene regulation in joint tissue engineering.

Hui (Herb) Sun, George M. Malacinski, Hiroki Yokota

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

We describe a new biochemical technique, "promoter competition assay," for examining the role of cis-acting DNA elements in tissue cultures. Recent advances in tissue engineering permit the culture of a variety of cells. Many tissues are engineered, however, without an appropriate understanding of molecular machinery that regulates gene expression and cellular growth. For elucidating the role of cis-acting regulatory elements in cellular differentiation and growth, we developed the promoter competition assay. This assay uses a transient transfer into cells of double-stranded DNA fragments consisting of cis-acting regulatory elements. The transferred DNA fragments act as a competitor and titrate the function of their genomic counterparts. Using synovial cells derived from a rheumatoid arthritis patient, we examined a role of NF-kappa B binding sites in the regulation of the expression of matrix metalloproteinase (MMP) genes. The results support a stimulatory role of NF-kappa B in transcriptional regulation of MMP-1 and MMP-13.

Original languageEnglish (US)
JournalFrontiers in Bioscience
Volume7
StatePublished - 2002
Externally publishedYes

Fingerprint

Tissue Engineering
Tissue engineering
Gene expression
Assays
NF-kappa B
Joints
DNA
Matrix Metalloproteinase 13
Genes
Tissue culture
Matrix Metalloproteinase 1
Growth
Matrix Metalloproteinases
Machinery
Rheumatoid Arthritis
Binding Sites
Tissue
Gene Expression

Cite this

Promoter competition assay for analyzing gene regulation in joint tissue engineering. / Sun, Hui (Herb); Malacinski, George M.; Yokota, Hiroki.

In: Frontiers in Bioscience, Vol. 7, 2002.

Research output: Contribution to journalArticle

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