Promoter-autonomous functioning in a controlled environment using single molecule FISH

Sami Hocine, Maria Vera, Daniel Zenklusen, Robert H. Singer

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Transcription is a highly regulated biological process, initiated through the assembly of complexes at the promoter that contain both the general transcriptional machinery and promoter-specific factors. Despite the abundance of studies focusing on transcription, certain questions have remained unanswered. It is not clear how the transcriptional profile of a promoter is affected by genomic context. Also, there is no single cell method to directly compare transcriptional profiles independent of gene length and sequence. In this work, we employ a single genetic site for isolating the transcriptional kinetics of yeast promoters. Utilizing single molecule FISH, we directly compare the transcriptional activity of different promoters, considering both synthesis and cell-to-cell variability. With this approach, we provide evidence suggesting promoters autonomously encode their associated transcriptional profiles, independent of genomic locus, gene length and gene sequence.

Original languageEnglish (US)
Article number9934
JournalScientific Reports
Volume5
DOIs
StatePublished - May 28 2015

ASJC Scopus subject areas

  • General

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