Prolyl isomerase pin1 acts downstream of mir200c to promote cancer stem-like cell traits in breast cancer

Man Li Luo, Chang Gong, Chun Hau Chen, Daniel Y. Lee, Hai Hu, Pengyu Huang, Yandan Yao, Wenjun Guo, Ferenc Reinhardt, Gerburg Wulf, Judy Lieberman, Xiao Zhen Zhou, Erwei Song, Kun Ping Lu

Research output: Contribution to journalArticle

39 Scopus citations

Abstract

Breast cancer stem-like cells (BCSC) have been implicated in tumor growth, metastasis, drug resistance, and relapse but druggable targets in appropriate subsets of this cell population have yet to be identified.Herewe identify a fundamental role for the prolyl isomerase Pin1 in driving BCSC expansion, invasiveness, and tumorigenicity, defining it as a key target of miR200c, which is known to be a critical regulator in BCSC. Pin1 overexpression expanded the growth and tumorigenicity of BCSC and triggered epithelial-mesenchymal transition. Conversely, genetic or pharmacological inhibition of Pin1 reduced the abundance and self-renewal activity of BCSC. Moreover, moderate overexpression of miR200c-resistant Pin1 rescued the BCSC defect in miR200c-expressing cells. Genetic deletion of Pin1 also decreased the abundance and repopulating capability of normal mouse mammary stem cells. In human cells, freshly isolated from reduction mammoplasty tissues, Pin1 overexpression endowed BCSC traits to normal breast epithelial cells, expanding both luminal and basal/myoepithelial lineages in these cells. In contrast, Pin1 silencing in primary breast cancer cells freshly isolated from clinical samples inhibited the expansion, self-renewal activity, and tumorigenesis of BCSC in vitro and in vivo. Overall, our work demonstrated that Pin1 is a pivotal regulator acting downstream of miR200c to drive BCSC and breast tumorigenicity, highlighting a new therapeutic target to eradicate BCSC. Cancer Res; 74(13); 3603-16.

Original languageEnglish (US)
Pages (from-to)3603-3616
Number of pages14
JournalCancer Research
Volume74
Issue number13
DOIs
StatePublished - Jul 1 2014
Externally publishedYes

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ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Luo, M. L., Gong, C., Chen, C. H., Lee, D. Y., Hu, H., Huang, P., Yao, Y., Guo, W., Reinhardt, F., Wulf, G., Lieberman, J., Zhou, X. Z., Song, E., & Lu, K. P. (2014). Prolyl isomerase pin1 acts downstream of mir200c to promote cancer stem-like cell traits in breast cancer. Cancer Research, 74(13), 3603-3616. https://doi.org/10.1158/0008-5472.CAN-13-2785