Proliferative response patterns of human fibroblasts after photoinjury with 4,5',8-trimethylpsoralen

Steven R. Cohen, D. M. Carter, M. Gala

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The extent of growth suppression and recovery following exposure to 4,5',8-trimethylpsoralen (TMP) plus UV-A irradiation was studied in 3 diploid human fibroblast strains. Inhibition of cellular proliferation was dose-dependent within the concentration range of TMP that was tested (1-4 x 10-7M), using a constant level of UV-A (0.24J/cm2). The population generation (doubling) times for all cell strains were progressively lengthened under these conditions while maximal cell densities were reduced. At 2-4 x 10-7M TMP in the presence of UV-A, there was a triphasic pattern of growth which consisted of (1) proliferative activity during the first 24-36 hr, followed by (2) complete growth inhibition for variable periods of time and (3) a recovery period of log phase proliferation that was not as vigorous as measured for untreated cells. There were also declines in the percentage of cells labeled with 3H-Tdr at various times after TMP-UV-A treatment. These measurements were essentially identical for the three fibroblast strains evaluated. In that the cells employed for these investigations were derived from embryonic pulmonary tissue, neonatal foreskin and the buttock skin of an adult male, it seems unlikely that donor age and tissue source were important variables in determining growth response patterns after TMP-UV-A exposure. Because proliferative recovery was attenuated after this photochemical injury, we conclude that the biologic effect(s) of TMP-UV-A extend beyond the known period of psoralen-DNA cross-link removal.

Original languageEnglish (US)
Pages (from-to)10-14
Number of pages5
JournalJournal of Investigative Dermatology
Volume76
Issue number1
StatePublished - 1981
Externally publishedYes

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Trioxsalen
Fibroblasts
Growth
Recovery
Tissue
Ficusin
Foreskin
Buttocks
Diploidy
Skin
Cell Count
Cell Proliferation
Tissue Donors
Irradiation
Lung
DNA
Wounds and Injuries

ASJC Scopus subject areas

  • Dermatology

Cite this

Proliferative response patterns of human fibroblasts after photoinjury with 4,5',8-trimethylpsoralen. / Cohen, Steven R.; Carter, D. M.; Gala, M.

In: Journal of Investigative Dermatology, Vol. 76, No. 1, 1981, p. 10-14.

Research output: Contribution to journalArticle

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