Proliferating cell nuclear antigen/cyclin is an interleukin 2-responsive gene.

K. S. Moore, K. Sullivan, E. M. Tan, Michael B. Prystowsky

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Previously, we have shown that a protein designated p36 is synthesized at a high rate during interleukin 2-driven proliferation of a cloned T lymphocyte, L2. Biosynthesis of p36 increases 1000-fold during the initial mid-G1 phase of the cell cycle and remains high while the cells proliferate. In this report, we show that p36 has the same migration pattern by two-dimensional gel electrophoresis as proliferating cell nuclear antigen (PCNA)/cyclin and that antiserum to PCNA/cyclin selectively immunoprecipitates p36. In addition, by indirect immunofluorescence, PCNA/cyclin accumulates in the nucleus of interleukin 2-stimulated L2 cells during proliferation and is not detectable prior to the initial S phase or after proliferation ceases. These data indicate that PCNA/cyclin expression is induced by interleukin 2 and that PCNA/cyclin accumulation is closely associated with T lymphocyte proliferation.

Original languageEnglish (US)
Pages (from-to)8447-8450
Number of pages4
JournalJournal of Biological Chemistry
Volume262
Issue number18
StatePublished - Jun 25 1987
Externally publishedYes

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Cyclins
Proliferating Cell Nuclear Antigen
Interleukin-2
Genes
T-cells
T-Lymphocytes
Biosynthesis
Electrophoresis, Gel, Two-Dimensional
Cell proliferation
G1 Phase
Indirect Fluorescent Antibody Technique
Electrophoresis
S Phase
Immune Sera
Cell Cycle
Gels
Cells
Cell Proliferation
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Proliferating cell nuclear antigen/cyclin is an interleukin 2-responsive gene. / Moore, K. S.; Sullivan, K.; Tan, E. M.; Prystowsky, Michael B.

In: Journal of Biological Chemistry, Vol. 262, No. 18, 25.06.1987, p. 8447-8450.

Research output: Contribution to journalArticle

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