TY - JOUR
T1 - Progress toward liquid biopsies in pediatric solid tumors
AU - Weiser, Daniel A.
AU - West-Szymanski, Diana C.
AU - Fraint, Ellen
AU - Weiner, Shoshana
AU - Rivas, Marco A.
AU - Zhao, Carolyn W.T.
AU - He, Chuan
AU - Applebaum, Mark A.
N1 - Publisher Copyright:
© 2019, Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2019/12/1
Y1 - 2019/12/1
N2 - Pediatric solid tumors have long been known to shed tumor cells, DNA, RNA, and proteins into the blood. Recent technological advances have allowed for improved capture and analysis of these typically scant circulating materials. Efforts are ongoing to develop “liquid biopsy” assays as minimally invasive tools to address diagnostic, prognostic, and disease monitoring needs in childhood cancer care. Applying these highly sensitive technologies to serial liquid biopsies is expected to advance understanding of tumor biology, heterogeneity, and evolution over the course of therapy, thus opening new avenues for personalized therapy. In this review, we outline the latest technologies available for liquid biopsies and describe the methods, pitfalls, and benefits of the assays that are being developed for children with extracranial solid tumors. We discuss what has been learned in several of the most common pediatric solid tumors including neuroblastoma, sarcoma, Wilms tumor, and hepatoblastoma and highlight promising future directions for the field.
AB - Pediatric solid tumors have long been known to shed tumor cells, DNA, RNA, and proteins into the blood. Recent technological advances have allowed for improved capture and analysis of these typically scant circulating materials. Efforts are ongoing to develop “liquid biopsy” assays as minimally invasive tools to address diagnostic, prognostic, and disease monitoring needs in childhood cancer care. Applying these highly sensitive technologies to serial liquid biopsies is expected to advance understanding of tumor biology, heterogeneity, and evolution over the course of therapy, thus opening new avenues for personalized therapy. In this review, we outline the latest technologies available for liquid biopsies and describe the methods, pitfalls, and benefits of the assays that are being developed for children with extracranial solid tumors. We discuss what has been learned in several of the most common pediatric solid tumors including neuroblastoma, sarcoma, Wilms tumor, and hepatoblastoma and highlight promising future directions for the field.
KW - Cell-free DNA
KW - Epigenetics
KW - Liquid biopsy
KW - Next-generation sequencing
KW - Pediatric solid tumors
UR - http://www.scopus.com/inward/record.url?scp=85076862009&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85076862009&partnerID=8YFLogxK
U2 - 10.1007/s10555-019-09825-1
DO - 10.1007/s10555-019-09825-1
M3 - Review article
C2 - 31836951
AN - SCOPUS:85076862009
SN - 0167-7659
VL - 38
SP - 553
EP - 571
JO - Cancer and Metastasis Reviews
JF - Cancer and Metastasis Reviews
IS - 4
ER -