Prognostic value of human papillomavirus types 16 and 18 DNA physical status in cervical intraepithelial neoplasia

A. Gradíssimo Oliveira; C. Delgado; N. Verdasca; Â Pista

doi:10.1111/1469-0691.12233

Prognostic value of human papillomavirus types 16 and 18 DNA physical status in cervical intraepithelial neoplasia

A. Gradíssimo Oliveira, C. Delgado, N. Verdasca, Â Pista

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

The aim of this work was to assess the value of the physical status of human papillomavirus (HPV) DNA as a disease marker for cervical cancer development in a set of 248 DNA samples previously genotyped as HPV 16 or 18, by calculating the E2/E6 ratio through real-time PCR. There was a significant difference in integration status according to disease grade for both genotypes (p <0.001). Furthermore, especially for HPV 18, determining the DNA physical status could be a useful biomarker in predicting cervical cancer risk development, with a lower E2/E6 ratio clinically associated with the development of a precancerous lesion.

Original language	English (US)
Pages (from-to)	E447-E450
Journal	Clinical Microbiology and Infection
Volume	19
Issue number	10
DOIs	https://doi.org/10.1111/1469-0691.12233
State	Published - Oct 2013
Externally published	Yes

Keywords

Cervical cancer
Human papillomavirus 18
Molecular marker
Physical status
Risk assessment

ASJC Scopus subject areas

Microbiology (medical)
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1111/1469-0691.12233

Cite this

@article{b19b8dc7d75a47e3bf957af58ae9e198,

title = "Prognostic value of human papillomavirus types 16 and 18 DNA physical status in cervical intraepithelial neoplasia",

abstract = "The aim of this work was to assess the value of the physical status of human papillomavirus (HPV) DNA as a disease marker for cervical cancer development in a set of 248 DNA samples previously genotyped as HPV 16 or 18, by calculating the E2/E6 ratio through real-time PCR. There was a significant difference in integration status according to disease grade for both genotypes (p <0.001). Furthermore, especially for HPV 18, determining the DNA physical status could be a useful biomarker in predicting cervical cancer risk development, with a lower E2/E6 ratio clinically associated with the development of a precancerous lesion.",

keywords = "Cervical cancer, Human papillomavirus 18, Molecular marker, Physical status, Risk assessment",

author = "{Grad{\'i}ssimo Oliveira}, A. and C. Delgado and N. Verdasca and {\^A} Pista",

note = "Funding Information: AGO is grateful to Funda{\c c}{\~a}o para a Ci{\v e}ncia e Tecnologia for a PhD studentship. The authors are very grateful to Rog{\'e}rio Tenreiro, PhD, at the Faculty of Sciences in the University of Lisbon for providing reagents to study the physical status. ",

year = "2013",

month = oct,

doi = "10.1111/1469-0691.12233",

language = "English (US)",

volume = "19",

pages = "E447--E450",

journal = "Clinical Microbiology and Infection",

issn = "1198-743X",

publisher = "Elsevier Limited",

number = "10",

}

TY - JOUR

T1 - Prognostic value of human papillomavirus types 16 and 18 DNA physical status in cervical intraepithelial neoplasia

AU - Gradíssimo Oliveira, A.

AU - Delgado, C.

AU - Verdasca, N.

AU - Pista, Â

N1 - Funding Information: AGO is grateful to Fundação para a Ciěncia e Tecnologia for a PhD studentship. The authors are very grateful to Rogério Tenreiro, PhD, at the Faculty of Sciences in the University of Lisbon for providing reagents to study the physical status.

PY - 2013/10

Y1 - 2013/10

N2 - The aim of this work was to assess the value of the physical status of human papillomavirus (HPV) DNA as a disease marker for cervical cancer development in a set of 248 DNA samples previously genotyped as HPV 16 or 18, by calculating the E2/E6 ratio through real-time PCR. There was a significant difference in integration status according to disease grade for both genotypes (p <0.001). Furthermore, especially for HPV 18, determining the DNA physical status could be a useful biomarker in predicting cervical cancer risk development, with a lower E2/E6 ratio clinically associated with the development of a precancerous lesion.

AB - The aim of this work was to assess the value of the physical status of human papillomavirus (HPV) DNA as a disease marker for cervical cancer development in a set of 248 DNA samples previously genotyped as HPV 16 or 18, by calculating the E2/E6 ratio through real-time PCR. There was a significant difference in integration status according to disease grade for both genotypes (p <0.001). Furthermore, especially for HPV 18, determining the DNA physical status could be a useful biomarker in predicting cervical cancer risk development, with a lower E2/E6 ratio clinically associated with the development of a precancerous lesion.

KW - Cervical cancer

KW - Human papillomavirus 18

KW - Molecular marker

KW - Physical status

KW - Risk assessment

UR - http://www.scopus.com/inward/record.url?scp=84884712407&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84884712407&partnerID=8YFLogxK

U2 - 10.1111/1469-0691.12233

DO - 10.1111/1469-0691.12233

M3 - Article

C2 - 23659555

AN - SCOPUS:84884712407

SN - 1198-743X

VL - 19

SP - E447-E450

JO - Clinical Microbiology and Infection

JF - Clinical Microbiology and Infection

IS - 10

ER -

Prognostic value of human papillomavirus types 16 and 18 DNA physical status in cervical intraepithelial neoplasia

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this