Production of embryonic and fetal-like red blood cells from human induced pluripotent stem cells

Chan Jung Chang, Koyel Mitra, Mariko Koya, Michelle Velho, Romain Desprat, Jack Lenz, Eric E. Bouhassira

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

We have previously shown that human embryonic stem cells can be differentiated into embryonic and fetal type of red blood cells that sequentially express three types of hemoglobins recapitulating early human erythropoiesis. We report here that we have produced iPS from three somatic cell types: adult skin fibroblasts as well as embryonic and fetal mesenchymal stem cells. We show that regardless of the age of the donor cells, the iPS produced are fully reprogrammed into a pluripotent state that is undistinguishable from that of hESCs by low and high-throughput expression and detailed analysis of globin expression patterns by HPLC. This suggests that reprogramming with the four original Yamanaka pluripotency factors leads to complete erasure of all functionally important epigenetic marks associated with erythroid differentiation regardless of the age or the tissue type of the donor cells, at least as detected in these assays. The ability to produce large number of erythroid cells with embryonic and fetal-like characteristics is likely to have many translational applications.

Original languageEnglish (US)
Article numbere25761
JournalPLoS One
Volume6
Issue number10
DOIs
StatePublished - Oct 13 2011

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Induced Pluripotent Stem Cells
Stem cells
Blood
erythrocytes
Erythrocytes
Cells
erythropoiesis
Globins
embryonic stem cells
Fibroblasts
Fetal Stem Cells
somatic cells
skin (animal)
epigenetics
fibroblasts
stem cells
Assays
hemoglobin
Skin
Erythroid Cells

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Production of embryonic and fetal-like red blood cells from human induced pluripotent stem cells. / Chang, Chan Jung; Mitra, Koyel; Koya, Mariko; Velho, Michelle; Desprat, Romain; Lenz, Jack; Bouhassira, Eric E.

In: PLoS One, Vol. 6, No. 10, e25761, 13.10.2011.

Research output: Contribution to journalArticle

Chang, Chan Jung ; Mitra, Koyel ; Koya, Mariko ; Velho, Michelle ; Desprat, Romain ; Lenz, Jack ; Bouhassira, Eric E. / Production of embryonic and fetal-like red blood cells from human induced pluripotent stem cells. In: PLoS One. 2011 ; Vol. 6, No. 10.
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