Production and purification of human menin from Drosophila melanogaster S2 cells using stirred tank reactor

M. A. Valle, M. B. Kester, A. L. Burns, S. J. Marx, A. M. Spiegel, J. Shiloach

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

A process was developed for producing human menin from transformed Drosophila Schneider 2 cells. Protein expression was achieved after inducing the metallothionein promoter by adding copper sulfate to cells growing in suspension in a stirred-tank reactor. Experiments in shake flasks showed that the production of menin was improved when the induction was conducted late in the exponential phase of cell growth at a concentration of 1-2×107cells ml-1, with a copper concentration of 0.2 mM for no more than 24 h. This observation was confirmed by experiments in bench-scale fermentors. Subsequently, a pilot-scale fermentation yielded 1 mg l-1 culture of purified menin.

Original languageEnglish (US)
Pages (from-to)127-135
Number of pages9
JournalCytotechnology
Volume35
Issue number2
DOIs
StatePublished - 2001

Keywords

  • Drosophila Schneider
  • Menin

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Clinical Biochemistry
  • Cell Biology

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