Production and purification of human menin from Drosophila melanogaster S2 cells using stirred tank reactor

M. A. Valle, M. B. Kester, A. L. Burns, S. J. Marx, Allen M. Spiegel, J. Shiloach

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

A process was developed for producing human menin from transformed Drosophila Schneider 2 cells. Protein expression was achieved after inducing the metallothionein promoter by adding copper sulfate to cells growing in suspension in a stirred-tank reactor. Experiments in shake flasks showed that the production of menin was improved when the induction was conducted late in the exponential phase of cell growth at a concentration of 1-2×107cells ml-1, with a copper concentration of 0.2 mM for no more than 24 h. This observation was confirmed by experiments in bench-scale fermentors. Subsequently, a pilot-scale fermentation yielded 1 mg l-1 culture of purified menin.

Original languageEnglish (US)
Pages (from-to)127-135
Number of pages9
JournalCytotechnology
Volume35
Issue number2
DOIs
StatePublished - 2001
Externally publishedYes

Fingerprint

Drosophila melanogaster
Purification
Copper Sulfate
Copper
Metallothionein
Cell growth
Fermentation
Suspensions
Experiments
Bioreactors
Proteins
Drosophila
Growth
Sulfates

Keywords

  • Drosophila Schneider
  • Menin

ASJC Scopus subject areas

  • Cell Biology
  • Biotechnology
  • Clinical Biochemistry

Cite this

Production and purification of human menin from Drosophila melanogaster S2 cells using stirred tank reactor. / Valle, M. A.; Kester, M. B.; Burns, A. L.; Marx, S. J.; Spiegel, Allen M.; Shiloach, J.

In: Cytotechnology, Vol. 35, No. 2, 2001, p. 127-135.

Research output: Contribution to journalArticle

Valle, M. A. ; Kester, M. B. ; Burns, A. L. ; Marx, S. J. ; Spiegel, Allen M. ; Shiloach, J. / Production and purification of human menin from Drosophila melanogaster S2 cells using stirred tank reactor. In: Cytotechnology. 2001 ; Vol. 35, No. 2. pp. 127-135.
@article{49bd3568e6304d9b934dc39946b199cc,
title = "Production and purification of human menin from Drosophila melanogaster S2 cells using stirred tank reactor",
abstract = "A process was developed for producing human menin from transformed Drosophila Schneider 2 cells. Protein expression was achieved after inducing the metallothionein promoter by adding copper sulfate to cells growing in suspension in a stirred-tank reactor. Experiments in shake flasks showed that the production of menin was improved when the induction was conducted late in the exponential phase of cell growth at a concentration of 1-2×107cells ml-1, with a copper concentration of 0.2 mM for no more than 24 h. This observation was confirmed by experiments in bench-scale fermentors. Subsequently, a pilot-scale fermentation yielded 1 mg l-1 culture of purified menin.",
keywords = "Drosophila Schneider, Menin",
author = "Valle, {M. A.} and Kester, {M. B.} and Burns, {A. L.} and Marx, {S. J.} and Spiegel, {Allen M.} and J. Shiloach",
year = "2001",
doi = "10.1023/A:1017586523710",
language = "English (US)",
volume = "35",
pages = "127--135",
journal = "Cytotechnology",
issn = "0920-9069",
publisher = "Springer Netherlands",
number = "2",

}

TY - JOUR

T1 - Production and purification of human menin from Drosophila melanogaster S2 cells using stirred tank reactor

AU - Valle, M. A.

AU - Kester, M. B.

AU - Burns, A. L.

AU - Marx, S. J.

AU - Spiegel, Allen M.

AU - Shiloach, J.

PY - 2001

Y1 - 2001

N2 - A process was developed for producing human menin from transformed Drosophila Schneider 2 cells. Protein expression was achieved after inducing the metallothionein promoter by adding copper sulfate to cells growing in suspension in a stirred-tank reactor. Experiments in shake flasks showed that the production of menin was improved when the induction was conducted late in the exponential phase of cell growth at a concentration of 1-2×107cells ml-1, with a copper concentration of 0.2 mM for no more than 24 h. This observation was confirmed by experiments in bench-scale fermentors. Subsequently, a pilot-scale fermentation yielded 1 mg l-1 culture of purified menin.

AB - A process was developed for producing human menin from transformed Drosophila Schneider 2 cells. Protein expression was achieved after inducing the metallothionein promoter by adding copper sulfate to cells growing in suspension in a stirred-tank reactor. Experiments in shake flasks showed that the production of menin was improved when the induction was conducted late in the exponential phase of cell growth at a concentration of 1-2×107cells ml-1, with a copper concentration of 0.2 mM for no more than 24 h. This observation was confirmed by experiments in bench-scale fermentors. Subsequently, a pilot-scale fermentation yielded 1 mg l-1 culture of purified menin.

KW - Drosophila Schneider

KW - Menin

UR - http://www.scopus.com/inward/record.url?scp=0034789356&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034789356&partnerID=8YFLogxK

U2 - 10.1023/A:1017586523710

DO - 10.1023/A:1017586523710

M3 - Article

C2 - 19003289

AN - SCOPUS:0034789356

VL - 35

SP - 127

EP - 135

JO - Cytotechnology

JF - Cytotechnology

SN - 0920-9069

IS - 2

ER -